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Mutational analysis of beta-catenin gene in Japanese ovarian carcinomas: frequent mutations in endometrioid carcinomas.

Abstract
To investigate the contribution of the beta-catenin gene to the development of ovarian carcinomas, mutational analysis of exon 3 of the beta-catenin gene was conducted. We analyzed 61 primary ovarian carcinomas, consisting of 49 non-endometrioid-type and 12 endometrioid-type tumors, for genetic alteration of the beta-catenin gene. Five carcinomas showed beta-catenin mutations (S37C, T41I, T41A), including 4 (33%) of 12 endometrioid-type tumors and 1 (14%) of 7 mucinous-type tumors. All of these mutations altered at the serine/threonine residues that are potential sites of GSK3-beta phosphorylation. We detected no carcinomas with interstitial deletion involving exon 3 of beta-catenin. Furthermore, we immunohistochemically studied 27 of the 61 ovarian carcinomas. Both nuclear and cytoplasmic beta-catenin expressions were demonstrated in 4 of the 27 ovarian carcinomas for which tissue samples were available for examination. All 4 cases exhibited mutations in exon 3 of beta-catenin, including a mucinous carcinoma. Our results suggested that beta-catenin gene mutation at potential GSK3-beta phosphorylation sites results in accumulation of beta-catenin protein within the cells and its translocation to nuclei. Accumulated beta-catenin protein may be involved in the development of endometrioid-type ovarian carcinomas, and some mucinous-type ovarian carcinomas.
AuthorsS Sagae, K Kobayashi, Y Nishioka, M Sugimura, S Ishioka, M Nagata, K Terasawa, T Tokino, R Kudo
JournalJapanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 90 Issue 5 Pg. 510-5 (May 1999) ISSN: 0910-5050 [Print] JAPAN
PMID10391090 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • CTNNB1 protein, human
  • Cytoskeletal Proteins
  • Trans-Activators
  • beta Catenin
Topics
  • Adult
  • Carcinoma, Endometrioid (genetics)
  • Cytoskeletal Proteins (genetics)
  • DNA Mutational Analysis
  • Female
  • Genome, Human
  • Humans
  • Immunohistochemistry
  • Japan
  • Middle Aged
  • Ovarian Neoplasms (genetics)
  • Trans-Activators
  • beta Catenin

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