Abstract | OBJECTIVE: The introduction of Euro-Collins solution with its intracellular electrolyte composition has allowed for clinically accepted pulmonary preservation for up to 7 h of ischemic time. In recent years several alternative solutions have been developed for the improvement of pulmonary preservation. Celsior is an extracellular solution which has significantly reduced the ischemia/reperfusion (IR)-induced pulmonary damage in animal studies. So far, no larger experimental studies exist concerning the influence of Celsior on pulmonary gas exchange following IR. METHODS: In an extracorporeal rat lung model ten lungs, were each preserved with Celsior (CE) and Celsior/ prostacycline (CEPC, 6 mg/100 ml) at 4 degrees C and compared with preservation with low- potassium- Euro-Collins solution (LPEC, 40 mmol/l of potassium). After 2 h of ischemia the lungs were re-ventilated and reperfused using a roller-pump. Relative oxygenation capacity (ROC), pulmonary vascular resistance (PVR), peak inspiratory pressure (PIP) and wet/dry ratio were monitored for 50 min. Statistical analysis was performed using ANOVA. RESULTS: ROC was increased in all Celsior preserved organs compared with the EC group (P < 0.032). Though the CEPC group was found to have the lowest PIP and the least amount of lung water as assessed by wet/dry ratio, PVR was highest after 30-50 min. The significantly lowest PVR was determined in the CE group (P < 0.02). CONCLUSIONS:
Celsior provides better lung preservation than LPEC solution, as demonstrated by a significantly increased oxygenation ability, a lower PVR and a decreased wet/ dry ratio. In vivo experiments and additional histological analysis are warranted for further evaluation of Celsior in lung preservation.
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Authors | T Wittwer, T Wahlers, J F Cornelius, S Elki, A Haverich |
Journal | European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery
(Eur J Cardiothorac Surg)
Vol. 15
Issue 5
Pg. 667-71
(May 1999)
ISSN: 1010-7940 [Print] Germany |
PMID | 10386415
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Celsior
- Disaccharides
- Electrolytes
- Glutamates
- Organ Preservation Solutions
- Mannitol
- Histidine
- Glutathione
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Topics |
- Analysis of Variance
- Animals
- Disaccharides
- Disease Models, Animal
- Electrolytes
- Extracorporeal Circulation
- Glutamates
- Glutathione
- Histidine
- Lung Transplantation
(methods, physiology)
- Male
- Mannitol
- Organ Preservation
(standards)
- Organ Preservation Solutions
- Oxygen Consumption
- Rats
- Rats, Sprague-Dawley
- Reference Values
- Sensitivity and Specificity
- Vascular Resistance
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