Abstract | BACKGROUND: METHODS AND RESULTS: Transcutaneous injection of recombinant adenovirus expressing Apobec1 (AvApobec1) into the liver of newborn rabbits in vivo resulted in efficient Apobec1 expression until Day 50, as detected by PCR-Southern blot analysis. By in vitro editing assay, liver extracts of AvApobec1-treated rabbits were found to have apoB mRNA editing activities of approximately 12, 15, and 15%, on Days 2, 10, and 20 after AvApobec1 administration, compared with 0% editing activity in AvLacZ control vector-injected animals. This physiological level of Apobec1 expression was associated with the production of apoB-48-containing lipoprotein particles from rabbit liver, with a concomitant 30% reduction in total plasma cholesterol compared to AvLacZ-treated or untreated control animals. CONCLUSION: Neonatal intrahepatic delivery of a first-generation adenoviral vector results in efficient gene transfer with little immune response, suggesting that repeated administration may be possible in the neonatal period.
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Authors | Y Wu, B B Teng, M L Brandt, P A Piedra, J Liu, L Chan |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 85
Issue 1
Pg. 148-57
(Jul 1999)
ISSN: 0022-4804 [Print] United States |
PMID | 10383852
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Copyright | Copyright 1999 Academic Press. |
Chemical References |
- Anticholesteremic Agents
- Recombinant Proteins
- Cholesterol
- AICDA (activation-induced cytidine deaminase)
- APOBEC-1 Deaminase
- Apobec1 protein, rat
- Cytidine Deaminase
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Topics |
- APOBEC-1 Deaminase
- Adenoviridae
(genetics, immunology)
- Aging
(immunology)
- Animals
- Animals, Newborn
(blood)
- Antibody Formation
(physiology)
- Anticholesteremic Agents
(metabolism)
- Cholesterol
(blood)
- Cytidine Deaminase
(genetics, immunology, physiology)
- Gene Transfer Techniques
- Genetic Vectors
(immunology)
- Liver
(physiology)
- Rabbits
- Rats
- Recombinant Proteins
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