Abstract |
CP-10 (chemotactic protein of m.w. 10,000) is a member of the S100 superfamily of Ca2+ binding peptides, which has potent chemotactic activity for murine and human myeloid cells. Here we report on the generation of monoclonal antibodies against CP-10 and accumulation of CP-10+ cells during experimental autoimmune encephalomyelitis (EAE), neuritis (EAN), uveitis (EAU) and in experimentally transplanted C6 gliomas. During acute inflammation, CP-10 is mainly expressed by large ED1+ monocytic perivascular cells that accumulate at days 11-14. CP-10+ cells are predominantly located in areas of cellular infiltration but are as well found in the meninges and infiltrating the brain parenchyma. In transplanted gliomas, CP-10+ cells are located exclusively within the tumor parenchyma. Using double labeling experiments, other cells participating in the inflammatory reaction were found to express CP-10, like few lymphoblastic W3/13+ cells in the vicinity of the inflammatory infiltrate.
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Authors | M H Deininger, Y Zhao, H J Schluesener |
Journal | Journal of neuroimmunology
(J Neuroimmunol)
Vol. 93
Issue 1-2
Pg. 156-63
(Jan 01 1999)
ISSN: 0165-5728 [Print] Netherlands |
PMID | 10378879
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Calgranulin A
- Chemotactic Factors
- DNA Primers
- Lymphocyte Function-Associated Antigen-1
- Macrophage-1 Antigen
- Recombinant Proteins
- S100 Proteins
- S100a8 protein, mouse
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Topics |
- Animals
- Antibodies, Monoclonal
- Autoimmune Diseases
(immunology)
- Base Sequence
- Calgranulin A
- Chemotactic Factors
(analysis, genetics, immunology)
- Cloning, Molecular
- DNA Primers
- Encephalomyelitis, Autoimmune, Experimental
(immunology)
- Flow Cytometry
- Gene Expression Regulation, Neoplastic
(immunology)
- Glioma
- Hybridomas
- Leukocytes
(chemistry, immunology)
- Lymphocyte Function-Associated Antigen-1
(analysis)
- Macrophage-1 Antigen
(analysis)
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Neuritis
(immunology)
- Rats
- Rats, Inbred Lew
- Recombinant Proteins
(immunology)
- S100 Proteins
(analysis, genetics, immunology)
- Spleen
(cytology)
- Tumor Cells, Cultured
(immunology)
- Uveitis
(immunology)
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