Inhibition of TGF-beta 3 restores the invasive capability of extravillous trophoblasts in preeclamptic pregnancies.

Abstract

Preeclampsia, the major cause of maternal morbidity and mortality in developed countries, is associated with abnormalities of placenta function due to shallow invasion of the maternal decidua by trophoblasts. Data suggest that TGF-beta may play a role in inhibiting trophoblast outgrowth or invasion, or both. We report that placental TGF-beta 3 expression is high in early pregnancy but falls at around 9 weeks' gestation. This pattern is inversely correlated with trophoblast outgrowth and fibronectin synthesis, markers of early trophoblast differentiation toward an invasive phenotype. We demonstrate that TGF-beta 3 is overexpressed in preeclamptic placentae. In contrast to control placentae, explants from preeclamptic pregnancies fail to exhibit spontaneous invasion in vitro. Significantly, antisense-induced inhibition of TGF-beta 3 expression, and inhibition of TGF-beta 3 activity with antibodies, induces the formation of columns of trophoblast cells, which migrate out of the explant into the underlying Matrigel. To our knowledge, this is the first demonstration that the hypoinvasive placental phenotype characteristic of preeclampsia can be essentially normalized in vitro by biochemical manipulation. We speculate that a failure to downregulate expression of TGF-beta 3 at around 9 weeks' gestation results in shallow trophoblast invasion and predisposes the pregnancy to preeclampsia.

• Authors: I Caniggia, S Grisaru-Gravnosky, M Kuliszewsky, M Post, S J Lye
• Journal: The Journal of clinical investigation (J Clin Invest) Vol. 103 Issue 12 Pg. 1641-50 (Jun 1999) ISSN: 0021-9738 [Print] United States
• PMID: 10377170 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protein Isoforms
• Transforming Growth Factor beta

Topics

• Cell Differentiation (genetics)
• Chorionic Villi (physiology)
• Down-Regulation (genetics)
• Extracellular Space (physiology)
• Female
• Humans
• Organ Culture Techniques
• Phenotype
• Pre-Eclampsia (metabolism, pathology, physiopathology)
• Pregnancy
• Pregnancy Trimester, First
• Protein Isoforms (biosynthesis, genetics)
• Transforming Growth Factor beta (antagonists & inhibitors, biosynthesis, genetics)
• Trophoblasts (cytology, physiology)