Hemolytic activity of copper sulfate as influenced by epinephrine and chelating thiols.

Abstract

AIM:

To study the effects of epinephrine, homocysteine, and other complexing agents on the cytotoxicity of copper sulfate.

METHODS:

In vitro suspensions of human red cells incubated with cupric sulfate were used, and hemolysis was determined by extracellular hemoglobin.

RESULTS:

The hemolytic activity of CuSO4 (0.3 mmol.L-1) was enhanced by the presence of epinephrine and to a lesser extent by homocysteine, whereas D-penicillamine, succimer, and mercaptodextran reduced the copper-induced hemolysis. The latter 3 chelating thiols also reduced the copper-epinephrine-induced hemolysis. The plasma protein ceruloplasmin reduced markedly the copper-epinephrine-induced hemolysis, even upon concentrations < 20% of that of copper. Chromic chloride, as well, acted anti-hemolytically.

CONCLUSION:

The latter protectors may interact with the production or activity of toxic oxygen, while classical copper chelators sequester cupric ions from interaction with epinephrine or homocysteine.

• Authors: J Aaseth, L G Korkina, I B Afanas'ev
• Journal: Zhongguo yao li xue bao = Acta pharmacologica Sinica (Zhongguo Yao Li Xue Bao) Vol. 19 Issue 3 Pg. 203-6 (May 1998) ISSN: 0253-9756 [Print] China
• PMID: 10375726 (Publication Type: Journal Article)

Chemical References

• Antidotes
• Chelating Agents
• Homocysteine
• Succimer
• Ceruloplasmin
• Penicillamine
• Copper Sulfate
• Epinephrine

Topics

• Adult
• Antidotes (toxicity)
• Ceruloplasmin (pharmacology)
• Chelating Agents (pharmacology)
• Copper Sulfate (toxicity)
• Drug Synergism
• Epinephrine (pharmacology)
• Hemolysis (drug effects)
• Homocysteine (pharmacology)
• Humans
• Penicillamine (pharmacology)
• Succimer (pharmacology)