Activated lansoprazole inhibits cancer cell adhesion to extracellular matrix components.

Abstract

Integrins play an important role in tumor dissemination. The purpose of this study was to examine whether a SH-reactive reagent inhibits the adhesion of the human pancreatic cancer cell line AsPC-1 to extracellular matrix components. Activated lansoprazole (AG-2000) was used as the SH-reactive reagent because this compound is known to react with SH groups but does not permeate the cell membrane. The effect of AG-2000 on the adhesion of AsPC-1 cells to matrix was examined, using both an in vitro adhesion assay and an in vivo nude mouse xenograft model of peritoneal implantation. In the in vitro adhesion assay, a 60-min exposure of AsPC-1 cells to AG-2000 resulted in a dose-dependent inhibition of AsPC-1 cell adhesion to laminin, fibronectin and type IV collagen, although AG-2000 did not affect the viability of AsPC-1 cells by MTT assay. In the in vivo assessment of AsPC-1 cell implantation, the AsPC-1 cells were initially preincubated with AG-2000 for 60 min to ensure adequate exposure of the AsPC-1 cells to AG-2000 before intraperitoneal injection. AG-2000 significantly inhibited the peritoneal implantation of the AsPC-1 cells in nude mice. These findings suggest that a short exposure of cancer cells to AG-2000 can inhibit cancer cell adhesion to extracellular matrix components.

• Authors: T Ohta, H Tajima, A Yachie, K Yokoyama, A Elnemr, S Fushida, H Kitagawa, M Kayahara, G Nishimura, K Miwa, M Yamamoto, T Terada, S Ohkuma
• Journal: International journal of oncology (Int J Oncol) Vol. 15 Issue 1 Pg. 33-9 (Jul 1999) ISSN: 1019-6439 [Print] Greece
• PMID: 10375591 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Benzimidazoles
• Pyridines
• Sulfhydryl Reagents
• AG 2000

Topics

• Animals
• Antineoplastic Agents (pharmacology, therapeutic use)
• Benzimidazoles (pharmacology, therapeutic use)
• Cell Adhesion (drug effects)
• Extracellular Matrix (metabolism)
• Humans
• Injections, Intraperitoneal
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Neoplastic Stem Cells (cytology, drug effects)
• Omentum
• Pancreatic Neoplasms (pathology)
• Peritoneal Neoplasms (prevention & control)
• Pyridines (pharmacology, therapeutic use)
• Sulfhydryl Reagents (pharmacology, therapeutic use)
• Tumor Cells, Cultured (drug effects)