Identification of peptides presented by HLA class I molecules on cervical cancer cells with HPV-18 infection.

Abstract

In this work we eluted peptides from purified class I MHC molecules, isolated from a novel human cervical carcinoma cell line (INBL), generated in our laboratory and positive for HPV-18 infection. A fraction of these peptides was capable of stimulating T lymphocytes obtained from a donor matched for HLA-Cw4 and who was also HPV-18+. Direct N-terminal Edman degradation of these peptides, revealed the sequence (XQFPIFLQF) that matched 85% with the sequence NVFPIFLQM localized in between the 54 and 62 residues of the HPV-18 L1 protein. After stimulation with the synthetic peptide NVFPIFLQM, T lymphocytes from the donor were capable to lyse INBL cells. Our results provide evidence of the existence of naturally occurring viral epitopes presented on cervical cancer cells by the HLA-Cw4 allele, that could be useful for immunotherapy on this type of patient.

• Authors: A M García, V F Ortíz-Navarrete, M L Mora-García, F Flores-Borja, A Diaz-Quiñonez, A Isibasi-Araujo, C Trejo-Becerril, R Chacón-Salinas, J Hernández-Montes, J Granados-Arreola, C de Leo, B Weiss-Steider
• Journal: Immunology letters (Immunol Lett) Vol. 67 Issue 3 Pg. 167-77 (Apr 15 1999) ISSN: 0165-2478 [Print] Netherlands
• PMID: 10369123 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Epitopes
• Histocompatibility Antigens Class I
• Peptides
• Viral Proteins

Topics

• Amino Acid Sequence
• Antigens, Viral (chemistry, immunology)
• Cytotoxicity, Immunologic
• Epitopes (immunology)
• Female
• Histocompatibility Antigens Class I (chemistry, immunology)
• Humans
• Lymphocyte Activation
• Mass Spectrometry
• Molecular Sequence Data
• Papillomaviridae (immunology)
• Papillomavirus Infections (immunology, virology)
• Peptides (chemistry, immunology, isolation & purification)
• T-Lymphocytes (immunology)
• Tumor Cells, Cultured
• Tumor Virus Infections (immunology, virology)
• Uterine Cervical Neoplasms (immunology, virology)
• Viral Proteins (chemistry, immunology, isolation & purification)