Synthesis of adenine and guanine nucleotides at the 'inosinic branch point' in lymphocytes of leukemia patients.

Abstract

The synthesis of purine nucleotides has been studied in human peripheral blood lymphocytes from healthy subjects and patients affected by B-cell chronic lymphocytic leukemia (B-CLL). The rate of the synthesis was measured by following the incorporation of 14C-formate into the nucleotides of lymphocyte suspensions. The whole sequence AMP-->ADP-->ATP was found reduced in B-CLL lymphocytes; in the case of guanylates only the last step of the sequence GMP-->GDP-->GTP was significantly lower in the same cells. From the analysis of these results, combined with previous data, we conclude that purine metabolism undergoes an imbalancement during CLL, which is partially compensated, and point out the importance of studying concomitantly purine metabolism and nucleic acid synthesis in leukemia cells.

• Authors: F Carlucci, A Tabucchi, R Pagani, E Marinello
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1454 Issue 1 Pg. 106-14 (May 31 1999) ISSN: 0006-3002 [Print] Netherlands
• PMID: 10354520 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Adenine Nucleotides
• Carbon Radioisotopes
• Guanine Nucleotides
• Inosine Monophosphate
• Adenosine Monophosphate
• Guanosine Monophosphate

Topics

• Adenine Nucleotides (biosynthesis)
• Adenosine Monophosphate (metabolism)
• Aged
• Carbon Radioisotopes
• Cells, Cultured
• Guanine Nucleotides (biosynthesis)
• Guanosine Monophosphate (metabolism)
• Humans
• Inosine Monophosphate (metabolism)
• Leukemia, Lymphocytic, Chronic, B-Cell (blood, metabolism)
• Lymphocytes (metabolism)
• Middle Aged
• Time Factors