A lysosomal storage disease induced by Ipomoea carnea in goats in Mozambique.

Abstract

A novel plant-induced lysosomal storage disease was observed in goats from a village in Mozambique. Affected animals were ataxic, with head tremors and nystagmus. Because of a lack of suitable feed, the animals consumed an exotic hedge plant growing in the village that was identified as Ipomoea carnea (shrubby morning glory, Convolvulaceae). The toxicosis was reproduced by feeding I. carnea plant material to goats. In acute cases, histologic changes in the brain and spinal cord comprised widespread cytoplasmic vacuolation of neurons and glial cells in association with axonal spheroid formation. Ultrastructurally, cytoplasmic storage vacuoles in neurons were membrane bound and consistent with lysosomes. Cytoplasmic vacuolation was also found in neurons in the submucosal and mesenteric plexuses in the small intestine, in renal tubular epithelial cells, and in macrophage-phagocytic cells in the spleen and lymph nodes in acute cases. Residual alterations in the brain in chronic cases revealed predominantly cerebellar lesions characterized by loss of Purkinje neurons and gliosis of the Purkinje cell layer. Analysis of I. carnea plant material by gas chromatography-mass spectrometry established the presence of the mannosidase inhibitor swainsonine and 2 glycosidase inhibitors, calystegine B2 and calystegine C1, consistent with a plant-induced alpha-mannosidosis in the goats. The described storage disorder is analogous to the lysosomal storage diseases induced by ingestion of locoweeds (Astragalus and Oxytropis) and poison peas (Swainsona).

• Authors: K K de Balogh, A P Dimande, J J van der Lugt, R J Molyneux, T W Naudé, W G Welman
• Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (J Vet Diagn Invest) Vol. 11 Issue 3 Pg. 266-73 (May 1999) ISSN: 1040-6387 [Print] United States
• PMID: 10353359 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• Nortropanes
• Solanaceous Alkaloids
• calystegine C1
• calystegine B(2)
• Swainsonine

Topics

• Animals
• Ataxia (etiology)
• Diet
• Disease Outbreaks
• Enzyme Inhibitors (analysis)
• Goat Diseases (pathology)
• Goats
• Lysosomal Storage Diseases (pathology, veterinary)
• Mozambique
• Nortropanes (analysis)
• Plant Poisoning (pathology, veterinary)
• Solanaceous Alkaloids
• Swainsonine (analysis)