Detailed transcript map of a 810-kb region at 11p14 involving identification of 10 novel human 3' exons.

Abstract

A limited number of genes, including the human brain-derived neutrotrophic factor (BDNF) gene, have been identified in the human chromosome 11p14 region. Since this area is involved in a genetic disorder (WAGR syndrome) and because of interest in studying the regulation of the human BDNF gene, we have established a detailed transcript map of a 810-kb region clone in a yeast artificial chromosome (YAC), corresponding to a portion of this genomic locus. A set of nested deletion mutants has been generated to map genes at a mean resolution of 75kb. Four genic markers from available mapping databases have been mapped on the YAC. Ten potential novel human exons have been isolated by a 3' terminal exon trapping procedure directly applied to purified YAC DNA. Most of these exons display polyadenylation signals and they all yield positive signals in RT-PCR experiments, confirming their status of transcribed sequences. The BDNF gene is now co-localised with three other genes on a 120 kb DNA fragment.

• Authors: F Guillemot, C Auffray, M D Devignes
• Journal: European journal of human genetics : EJHG (Eur J Hum Genet) 1999 May-Jun Vol. 7 Issue 4 Pg. 487-95 ISSN: 1018-4813 [Print] England
• PMID: 10352939 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Brain-Derived Neurotrophic Factor

Topics

• Brain-Derived Neurotrophic Factor (genetics)
• Chromosome Mapping
• Chromosomes, Artificial, Yeast
• Chromosomes, Human, Pair 11 (genetics)
• Electrophoresis, Gel, Pulsed-Field
• Exons (genetics)
• Humans
• Microsatellite Repeats (genetics)
• Molecular Sequence Data
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Analysis, DNA
• Transcription, Genetic
• WAGR Syndrome (genetics)