Insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) is a potential tumor suppressor protein for prostate cancer.

Abstract

Insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1) has been shown to have decreased expression in the progression from benign to malignant prostate epithelial cells (V. Hwa et al., J. Clin Endocrinol. Metab., 83: 4355-4362, 1998). The present study was undertaken to determine the effects of the re-expression of IGFBP-rP1 in a cell line from a model of human prostate cancer, M12, in which IGFBP-rP1 expression had been demonstrated to decrease from the parent epithelial cell, P69, to the malignant subline, M12. An IGFBP-rP1 cDNA encoding the protein was transfected into M12 cells in a plasmid that resulted in constitutive-expression of IGFBP-rP1. Clones of transfected M12 cells were selected for low (L) and high (H) levels of expression, and the plasmid vector alone was transfected into M12 as a control. After transfection, there was a marked alteration in the morphology of the M12 cells such that the H clones had an elongated appearance when compared with the M12 control cells. The M12 clones overexpressing IGFBP-rP1 had a dose-related increase in population doubling time, decreased colony formation in soft agar, an increased propensity to undergo apoptosis in response to 6-hydroxyurea, and decreased tumor formation in male athymic, nude mice. These data suggest that IGFBP-rP1 may have a suppressive effect on prostate cancer development.

• Authors: C C Sprenger, S E Damon, V Hwa, R G Rosenfeld, S R Plymate
• Journal: Cancer research (Cancer Res) Vol. 59 Issue 10 Pg. 2370-5 (May 15 1999) ISSN: 0008-5472 [Print] United States
• PMID: 10344746 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Carrier Proteins
• DNA, Complementary
• Insulin-Like Growth Factor Binding Proteins
• Neoplasm Proteins
• Recombinant Fusion Proteins
• insulin-like growth factor binding protein-related protein 1
• Hydroxyurea

Topics

• Animals
• Apoptosis (drug effects)
• Carrier Proteins (biosynthesis, genetics, physiology)
• Cell Division
• Cell Size
• DNA, Complementary (genetics)
• Genes, Tumor Suppressor
• Humans
• Hydroxyurea (pharmacology)
• Insulin-Like Growth Factor Binding Proteins (biosynthesis, genetics, physiology)
• Male
• Mice
• Mice, Nude
• Neoplasm Proteins (biosynthesis, genetics, physiology)
• Neoplasm Transplantation
• Prostatic Neoplasms (genetics, pathology)
• Recombinant Fusion Proteins (physiology)
• Transfection
• Tumor Cells, Cultured
• Tumor Stem Cell Assay