HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Teneurin-1, a vertebrate homologue of the Drosophila pair-rule gene ten-m, is a neuronal protein with a novel type of heparin-binding domain.

Abstract
The Drosophila gene ten-m is the first pair-rule gene not encoding a transcription factor, but an extracellular protein. We have characterized a highly conserved chicken homologue that we call teneurin-1. The C-terminal part harbors 26 repetitive sequence motifs termed YD-repeats. The YD-repeats are most similar to the core of the rhs elements of Escherichia coli. Related repeats in toxin A of Clostridium difficile are known to bind specific carbohydrates. We show that recombinantly expressed proteins containing the YD-repeats of teneurin-1 bind to heparin. Furthermore, heparin lyase treatment of extracts of cells expressing recombinant YD-repeat protein releases this protein from high molecular mass aggregates. In situ hybridization and immunostaining reveals teneurin-1 expression in neurons of the developing visual system of chicken and Drosophila. This phylogenetic conservation of neuronal expression from flies to birds implies fundamental roles for teneurin-1 in neurogenesis. This is supported by the neurite outgrowth occurring on substrates made of recombinant YD-repeat proteins, which can be inhibited by heparin. Database searches resulted in the identification of ESTs encoding at least three further members of the teneurin family of proteins. Furthermore, the human teneurin-1 gene could be identified on chromosome Xq24/25, a region implied in an X-linked mental retardation syndrome.
AuthorsA D Minet, B P Rubin, R P Tucker, S Baumgartner, R Chiquet-Ehrismann
JournalJournal of cell science (J Cell Sci) Vol. 112 ( Pt 12) Pg. 2019-32 (Jun 1999) ISSN: 0021-9533 [Print] England
PMID10341219 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Nerve Tissue Proteins
  • Recombinant Proteins
  • Tenascin
  • teneurin-1
  • Heparin
Topics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caenorhabditis elegans (genetics)
  • Chick Embryo
  • Chickens (genetics)
  • Drosophila (genetics)
  • Heparin (metabolism)
  • Humans
  • Molecular Sequence Data
  • Nerve Tissue Proteins (genetics)
  • Neurons (metabolism)
  • Phylogeny
  • Protein Binding
  • Protein Structure, Tertiary
  • Recombinant Proteins (metabolism)
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Tenascin (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: