Abstract |
The aim of the present study was to evaluate the action of plasma from insulin-dependent diabetic ( IDDM) pregnant women on nitric oxide synthase (NOS) activity in cultured human umbilical vein endothelial cells (HUVECs). We also studied the effect of the plasma on cytosolic calcium and on Na+/K+- adenosine triphosphatase ( ATPase) activity. Dynamic fluorescence studies of membrane fluidity were contemporarily performed to detect a direct effect of plasma on the endothelial cell membrane. We observed a significant increase in NOS activity, intracellular calcium, and Na+/K+- ATPase activity in cultured HUVECs exposed to IDDM plasma. Our dynamic fluorescence study showed a different microenvironmental organization of the cellular membrane after incubation with plasma from IDDM pregnant women, with a marked decrease in microheterogeneity as evaluated in terms of 1-(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene ( TMA-DPH) lifetime distribution width. The present investigation suggests that plasma from IDDM pregnant women can cause a generalized disturbance in the function of endothelial cells cultured from healthy subjects. Such a modification might play a central role in the pathogenesis of the vascular complications of the disease.
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Authors | E Salvolini, R A Rabini, D Martarelli, N Moretti, N Cester, L Mazzanti |
Journal | Metabolism: clinical and experimental
(Metabolism)
Vol. 48
Issue 5
Pg. 554-7
(May 1999)
ISSN: 0026-0495 [Print] United States |
PMID | 10337852
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fluorescent Dyes
- Diphenylhexatriene
- 1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene
- NOS3 protein, human
- Nitric Oxide Synthase
- Nitric Oxide Synthase Type III
- Sodium-Potassium-Exchanging ATPase
- Calcium
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Topics |
- Adult
- Blood Physiological Phenomena
- Calcium
(metabolism)
- Cell Membrane
(physiology)
- Cells, Cultured
- Cytosol
(metabolism)
- Diabetes Mellitus, Type 1
(blood)
- Diphenylhexatriene
(analogs & derivatives)
- Endothelium, Vascular
(cytology, metabolism)
- Female
- Fluorescent Dyes
- Fluorometry
- Humans
- Membrane Fluidity
(physiology)
- Nitric Oxide Synthase
(metabolism)
- Nitric Oxide Synthase Type III
- Pregnancy
- Pregnancy in Diabetics
(blood)
- Sodium-Potassium-Exchanging ATPase
(metabolism)
- Umbilical Veins
(cytology, metabolism)
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