Modulating activity of indomethacin to vincristine cytotoxicity in various human carcinoma cells.

The modulating activity of indomethacin to vincristine (VCR) was investigated in thirty human pulmonary carcinoma cell lines (adenocarcinoma 9, large cell carcinoma 9, squamous cell carcinoma 6, small cell carcinoma 6) and five other cell lines (colon carcinoma 2, melanoma 1, teratocarcinoma 1, thymoma 1). The survival of these cell lines treated with VCR with or without indomethacin (2 microg/ml) for 72 hours were examined using MTT assay, and IC50 values were calculated. When the cutoff level of potent combined effect in clinical use was set at 2-fold increase of sensitivity, the positive rate was 100% for adenocarcinomas and large cell carcinomas, 25% for squamous cell carcinomas, 33% for small cell carcinomas. Mean modulating index was 2.91 in adenocarcinomas, 1.92 in squamous cell carcinomas, 3.06 in large cell carcinomas and 1.67 in small cell carcinomas. Of the cell lines of other tumors, three cell lines (colon carcinoma 1, melanoma 1, teratocarcinoma 1) showed the potent combined effect of VCR and indomethacin, while indomethacin was not effective in modulating activity to VCR in a thymoma cell line and fibroblast cells. In conclusion, it is considered that modulating activity of indomethacin for VCR is a general effect for various human cancer cells, and combined use with VCR and indomethacin may be a useful modulation therapy for the advanced lung cancer.
AuthorsS Kobayashi, S Okada, T Hasumi, N Sato, S Fujimura
JournalThe Tohoku journal of experimental medicine (Tohoku J Exp Med) Vol. 186 Issue 4 Pg. 313-22 (Dec 1998) ISSN: 0040-8727 [Print] JAPAN
PMID10328163 (Publication Type: Journal Article)
Chemical References
  • Anti-Inflammatory Agents, Non-Steroidal
  • Antineoplastic Agents, Phytogenic
  • Coloring Agents
  • Tetrazolium Salts
  • Thiazoles
  • thiazolyl blue
  • Vincristine
  • Indomethacin
  • Adult
  • Aged
  • Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
  • Antineoplastic Agents, Phytogenic (antagonists & inhibitors, toxicity)
  • Carcinoma (pathology)
  • Cell Survival (drug effects)
  • Coloring Agents
  • Female
  • Fibroblasts
  • Humans
  • Indomethacin (pharmacology)
  • Lung Neoplasms (pathology)
  • Male
  • Middle Aged
  • Neoplasms (pathology)
  • Tetrazolium Salts
  • Thiazoles
  • Tumor Cells, Cultured
  • Vincristine (antagonists & inhibitors, toxicity)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: