Abstract | BACKGROUND: OBJECTIVE: METHODS: Forty-two (n = 42) patients with severe cystic acne had liver function tests, lipid estimations, serum biotin as well as biotinidase activity evaluations before (value 1) and on the 30th day (value 2) of treatment with isotretinoin monotherapy ( Roaccutane 0.5 mg/kg/24 h). The same laboratory tests were evaluated in 50 controls only once. Moreover, the effect of isotretinoin on a known plasma biotinidase activity was evaluated after incubation in vitro with various concentrations of the drug. RESULTS: A statistically significant elevation of liver enzymes and lipids, except high density lipoprotein, was observed at the end of this study. On the contrary, biotinidase activity was found to be significantly decreased as compared to the initial values (value 1 = 4.70 +/- 0.89 nmol/min/l, value 2 = 2.50 +/- 0.8 nmol/min/l, p < 0.001) and to controls (5.2 +/- 0.9 nmol/min/l vs. value 2 = 2.50 +/- 0.8 nmol/min/l, p < 0.001). Additionally, biotin levels showed no significant alterations and the in vitro incubation of the enzyme with various concentrations of the drug exhibited no effect on its activity. CONCLUSION:
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Authors | K H Schulpis, S Georgala, E D Papakonstantinou, T Michas, G A Karikas |
Journal | Skin pharmacology and applied skin physiology
(Skin Pharmacol Appl Skin Physiol)
1999 Jan-Apr
Vol. 12
Issue 1-2
Pg. 28-33
ISSN: 1422-2868 [Print] Switzerland |
PMID | 10325581
(Publication Type: Clinical Trial, Journal Article)
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Chemical References |
- Lipids
- Biotin
- Amidohydrolases
- Biotinidase
- Isotretinoin
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Topics |
- Acne Vulgaris
(drug therapy)
- Amidohydrolases
(blood, metabolism)
- Biotin
(metabolism)
- Biotinidase
- Dose-Response Relationship, Drug
- Female
- Fluorometry
- Humans
- Isotretinoin
(adverse effects)
- Lipids
(blood)
- Liver
(drug effects, enzymology)
- Liver Function Tests
- Male
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