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High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 are significant target antigens of perinuclear anti-neutrophil cytoplasmic antibodies in autoimmune hepatitis.

AbstractBACKGROUND:
High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 have been identified as novel antigens of perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCAs), and the existence of anti-HMG1 and anti-HMG2 antibodies in a population of patients with ulcerative colitis has been reported.
AIMS:
To investigate whether HMG1 and HMG2 are target antigens for p-ANCAs in autoimmune hepatitis (AIH).
PATIENTS:
Serum samples from 28 patients with AIH, 44 patients with primary biliary cirrhosis (PBC), 27 patients with chronic hepatitis C, and 23 patients with chronic hepatitis B were tested.
METHODS:
ANCAs were detected by routine indirect immunofluorescence (IIF). Anti-HMG1 and anti-HMG2 antibodies were assayed by enzyme linked immunosorbent assay.
RESULTS:
p-ANCAs were detected in 89% (25/28) of patients with AIH, 36% (16/44) of patients with PBC, 11% (3/27) of patients with chronic hepatitis C, and 13% (3/23) of patients with chronic hepatitis B. Anti-HMG1 and/or anti-HMG2 antibodies were detected in 89% (25/28) of patients with AIH, 70% (31/44) with PBC, 26% (7/27) with chronic hepatitis C, and 9% (2/23) with chronic hepatitis B. In AIH, anti-HMG1 and/or anti-HMG2 antibodies were detected in 96% (24/25) of p-ANCA positive patients. The p-ANCA staining pattern detected by IIF using sera from patients with AIH disappeared or decreased in titre after preincubation with a mixture of HMG1/HMG2. The presence and titres of those antibodies in AIH correlated significantly with those of p-ANCA, but not with those of anti-nuclear antibody or anti-smooth muscle antibody.
CONCLUSIONS:
HMG1 and HMG2 are significant target antigens of p-ANCA in AIH.
AuthorsJ Sobajima, S Ozaki, H Uesugi, F Osakada, M Inoue, Y Fukuda, H Shirakawa, M Yoshida, A Rokuhara, H Imai, K Kiyosawa, K Nakao
JournalGut (Gut) Vol. 44 Issue 6 Pg. 867-73 (Jun 1999) ISSN: 0017-5749 [Print] England
PMID10323891 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Antineutrophil Cytoplasmic
  • Autoantibodies
  • High Mobility Group Proteins
  • Cathepsins
  • Lactoferrin
  • Serine Endopeptidases
  • CTSG protein, human
  • Cathepsin G
Topics
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Antineutrophil Cytoplasmic (immunology)
  • Autoantibodies (analysis)
  • Cathepsin G
  • Cathepsins (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Hepatitis B, Chronic (immunology)
  • Hepatitis C, Chronic (immunology)
  • Hepatitis, Autoimmune (immunology)
  • High Mobility Group Proteins (immunology)
  • Humans
  • Lactoferrin (immunology)
  • Liver Cirrhosis, Biliary (immunology)
  • Male
  • Middle Aged
  • Serine Endopeptidases
  • Statistics, Nonparametric

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