Abstract | BACKGROUND: AIMS: PATIENTS: METHODS: ANCAs were detected by routine indirect immunofluorescence (IIF). Anti-HMG1 and anti-HMG2 antibodies were assayed by enzyme linked immunosorbent assay. RESULTS: p-ANCAs were detected in 89% (25/28) of patients with AIH, 36% (16/44) of patients with PBC, 11% (3/27) of patients with chronic hepatitis C, and 13% (3/23) of patients with chronic hepatitis B. Anti-HMG1 and/or anti-HMG2 antibodies were detected in 89% (25/28) of patients with AIH, 70% (31/44) with PBC, 26% (7/27) with chronic hepatitis C, and 9% (2/23) with chronic hepatitis B. In AIH, anti-HMG1 and/or anti-HMG2 antibodies were detected in 96% (24/25) of p-ANCA positive patients. The p-ANCA staining pattern detected by IIF using sera from patients with AIH disappeared or decreased in titre after preincubation with a mixture of HMG1/ HMG2. The presence and titres of those antibodies in AIH correlated significantly with those of p-ANCA, but not with those of anti-nuclear antibody or anti-smooth muscle antibody. CONCLUSIONS:
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Authors | J Sobajima, S Ozaki, H Uesugi, F Osakada, M Inoue, Y Fukuda, H Shirakawa, M Yoshida, A Rokuhara, H Imai, K Kiyosawa, K Nakao |
Journal | Gut
(Gut)
Vol. 44
Issue 6
Pg. 867-73
(Jun 1999)
ISSN: 0017-5749 [Print] England |
PMID | 10323891
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Antineutrophil Cytoplasmic
- Autoantibodies
- High Mobility Group Proteins
- Cathepsins
- Lactoferrin
- Serine Endopeptidases
- CTSG protein, human
- Cathepsin G
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Antibodies, Antineutrophil Cytoplasmic
(immunology)
- Autoantibodies
(analysis)
- Cathepsin G
- Cathepsins
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Female
- Fluorescent Antibody Technique, Indirect
- Hepatitis B, Chronic
(immunology)
- Hepatitis C, Chronic
(immunology)
- Hepatitis, Autoimmune
(immunology)
- High Mobility Group Proteins
(immunology)
- Humans
- Lactoferrin
(immunology)
- Liver Cirrhosis, Biliary
(immunology)
- Male
- Middle Aged
- Serine Endopeptidases
- Statistics, Nonparametric
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