Abstract |
We have identified T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium tumefaciens (rat mutants). These mutants are highly recalcitrant to the induction of both crown gall tumors and phosphinothricin-resistant calli. The results of transient GUS ( beta-glucuronidase) assays suggest that some of these mutants are blocked at an early step in the Agrobacterium-mediated transformation process, whereas others are blocked at a step subsequent to translocation of T-DNA into the nucleus. Attachment of Agrobacterium to roots of the mutants rat1 and rat3 was decreased under various incubation conditions. In most mutants, the transformation-deficient phenotype co-segregated with the kanamycin resistance encoded by the mutagenizing T-DNA. In crosses with susceptible wild-type plants, the resistance phenotype of many of these mutants segregated either as a semi-dominant or dominant trait.
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Authors | J Nam, K S Mysore, C Zheng, M K Knue, A G Matthysse, S B Gelvin |
Journal | Molecular & general genetics : MGG
(Mol Gen Genet)
Vol. 261
Issue 3
Pg. 429-38
(Apr 1999)
ISSN: 0026-8925 [Print] Germany |
PMID | 10323222
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
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Topics |
- Agrobacterium tumefaciens
(genetics, physiology)
- Arabidopsis
(genetics, microbiology)
- Bacterial Adhesion
- Crosses, Genetic
- DNA, Bacterial
(genetics)
- Genes, Plant
- Mutation
- Plant Roots
(microbiology)
- Transformation, Genetic
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