Abstract | BACKGROUND/AIMS: The aim of this work is to evaluate the repair of mucosal epithelium at the critical time of the beginning of reepithelization of stomach wound. METHODOLOGY: Structural and ultrastructural evaluation of rat gastric mucosa repair was carried out with administration of placebo, cimetidine and pentagastrin in different groups of rats (Rattus norvegicus albinus) during the first 7 post-operative days. Following 48 hours of starvation and anesthesia by ethyl ether inhalation, the animals were submitted to laparotomy exposing the stomach. A fusiform gastric resection measuring 5 mm in length was performed in the fundus stomach wall. The surgical wound was sutured in 2 plans: muscular tunic and visceral serosa. The abdominal wall was also sutured in 2 plans: parietal serosa-abdominal musculature and skin. During 6 consecutive post-operative days, the placebo group rats received 1 ml of distilled water intragastrically; those of the cimetidine group 10 mg/kg of cimetidine; and, animals of the pentagastrin group, 250 micrograms/kg of pentagastrin by intraperitoneal injection. On the 7th post-operative day, rats were killed and fragments of the stomach wall containing transverse section of surgical wound were collected and processed for light microscopy and electron microscopies. RESULTS: CONCLUSIONS: Comparative mucosal morphology showed that cimetidine tends to accelerate the beginning of reepithelization of granulation tissue while pentagastrin delays reepithelization due to alteration of protective mucus and a provoking increase of granulation tissue.
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Authors | M Giovanoni, C Hayashi, H Hayashi, E Haapalainen, S Geocze |
Journal | Hepato-gastroenterology
(Hepatogastroenterology)
1999 Jan-Feb
Vol. 46
Issue 25
Pg. 574-83
ISSN: 0172-6390 [Print] Greece |
PMID | 10228864
(Publication Type: Journal Article)
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Chemical References |
- Histamine H2 Antagonists
- Cimetidine
- Pentagastrin
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Topics |
- Animals
- Cimetidine
(pharmacology)
- Epithelial Cells
(pathology)
- Gastric Mucosa
(pathology)
- Granulation Tissue
(drug effects)
- Histamine H2 Antagonists
(pharmacology)
- Male
- Pentagastrin
(pharmacology)
- Rats
- Rats, Wistar
- Wound Healing
(drug effects)
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