Activation of extracellular signal-regulated kinases 1 and 2 by insulin-like growth factor I in human osteosarcoma MG-63 cells.

Abstract

The activation of extracellular signal-regulated kinases 1 and 2 by insulin-like growth factor I in human osteosarcoma MG-63 cells was examined by Mono Q ion exchange chromatography of cell extracts and measurement of myelin basic protein kinase activity, and by immunoblotting of cell extracts with a phospho-specific extracellular signal-regulated kinase antibody. Extracellular signal-regulated kinase 1 appeared to be activated in resting cells and addition of insulin-like growth factor I resulted in the activation primarily of extracellular signal-regulated kinase 2. Extracellular signal-regulated kinase 2 was found in the nucleus after addition of insulin-like growth factor I.

• Authors: W Lopaczynski, S Harris, P Nissley
• Journal: Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme (Horm Metab Res) 1999 Feb-Mar Vol. 31 Issue 2-3 Pg. 65-9 ISSN: 0018-5043 [Print] Germany
• PMID: 10226783 (Publication Type: Journal Article)

Chemical References

• Insulin-Like Growth Factor I
• Receptor, IGF Type 1
• Calcium-Calmodulin-Dependent Protein Kinases
• Mitogen-Activated Protein Kinase 1
• Mitogen-Activated Protein Kinase 3
• Mitogen-Activated Protein Kinases

Topics

• Calcium-Calmodulin-Dependent Protein Kinases (metabolism)
• Cell Nucleus (enzymology)
• Chromatography, High Pressure Liquid
• Fibroblasts (cytology, enzymology)
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Neoplastic
• Humans
• Insulin-Like Growth Factor I (physiology)
• Mitogen-Activated Protein Kinase 1
• Mitogen-Activated Protein Kinase 3
• Mitogen-Activated Protein Kinases
• Osteosarcoma
• Receptor, IGF Type 1 (genetics)
• Signal Transduction (physiology)
• Tumor Cells, Cultured (enzymology)