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Serum galactosyl hydroxylysine as a biochemical marker of bone resorption.

AbstractBACKGROUND:
Serum-based biochemical markers of bone resorption may provide better clinical information than urinary markers because direct comparison with serum markers of bone formation is possible and because the within-subject variability of serum markers may be lower. We describe a method for the measurement of free beta-1-galactosyl-O-hydroxylysine (Gal-Hyl) in serum.
METHODS:
The assay used preliminary ultrafiltration of serum, dansylation, and separation by reversed-phase HPLC with fluorescence detection. Healthy subjects were recruited from population-based studies of bone turnover.
RESULTS:
The within-run (n = 15) and between-run (n = 15) CVs were 7% and 14%, respectively, at a mean value of 48 nmol/L. In women and pubertal girls, serum free Gal-Hyl correlated with urine free Gal-Hyl (r = 0.84; P <0.001). Serum Gal-Hyl was higher during puberty and increased after menopause. The fractional renal clearance of free Gal-Hyl relative to that of creatinine was 0.90 (95% confidence interval, 0.82-0.98). Serum free Gal-Hyl decreased by 36% (SE = 4%) in 14 patients with mild Paget disease treated with an oral bisphosphonate, and this decrease was significantly (P <0. 001) greater than that seen for either serum tartrate-resistant acid phosphatase (9%; SE = 4%) or serum C-terminal telopeptide of collagen I (19%; SE = 8%).
CONCLUSION:
Serum free Gal-Hyl may be useful as a serum marker of bone resorption.
AuthorsA W Al-Dehaimi, A Blumsohn, R Eastell
JournalClinical chemistry (Clin Chem) Vol. 45 Issue 5 Pg. 676-81 (May 1999) ISSN: 0009-9147 [Print] England
PMID10222355 (Publication Type: Clinical Trial, Journal Article)
CopyrightCopyright 1999 American Association for Clinical Chemistry.
Chemical References
  • Biomarkers
  • Diphosphonates
  • Hydroxylysine
  • galactosylhydroxylysine
Topics
  • Adolescent
  • Adult
  • Age Factors
  • Aged
  • Aged, 80 and over
  • Biomarkers (blood, urine)
  • Bone Resorption (blood, urine)
  • Child
  • Diphosphonates (therapeutic use)
  • Female
  • Humans
  • Hydroxylysine (analogs & derivatives, blood, urine)
  • Menopause
  • Middle Aged
  • Osteitis Deformans (drug therapy, urine)
  • Puberty

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