Abstract | BACKGROUND: METHODS: The assay used preliminary ultrafiltration of serum, dansylation, and separation by reversed-phase HPLC with fluorescence detection. Healthy subjects were recruited from population-based studies of bone turnover. RESULTS: The within-run (n = 15) and between-run (n = 15) CVs were 7% and 14%, respectively, at a mean value of 48 nmol/L. In women and pubertal girls, serum free Gal-Hyl correlated with urine free Gal-Hyl (r = 0.84; P <0.001). Serum Gal-Hyl was higher during puberty and increased after menopause. The fractional renal clearance of free Gal-Hyl relative to that of creatinine was 0.90 (95% confidence interval, 0.82-0.98). Serum free Gal-Hyl decreased by 36% (SE = 4%) in 14 patients with mild Paget disease treated with an oral bisphosphonate, and this decrease was significantly (P <0. 001) greater than that seen for either serum tartrate-resistant acid phosphatase (9%; SE = 4%) or serum C-terminal telopeptide of collagen I (19%; SE = 8%). CONCLUSION:
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Authors | A W Al-Dehaimi, A Blumsohn, R Eastell |
Journal | Clinical chemistry
(Clin Chem)
Vol. 45
Issue 5
Pg. 676-81
(May 1999)
ISSN: 0009-9147 [Print] England |
PMID | 10222355
(Publication Type: Clinical Trial, Journal Article)
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Copyright | Copyright 1999 American Association for Clinical Chemistry. |
Chemical References |
- Biomarkers
- Diphosphonates
- Hydroxylysine
- galactosylhydroxylysine
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Topics |
- Adolescent
- Adult
- Age Factors
- Aged
- Aged, 80 and over
- Biomarkers
(blood, urine)
- Bone Resorption
(blood, urine)
- Child
- Diphosphonates
(therapeutic use)
- Female
- Humans
- Hydroxylysine
(analogs & derivatives, blood, urine)
- Menopause
- Middle Aged
- Osteitis Deformans
(drug therapy, urine)
- Puberty
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