Abstract |
Cellular adhesion receptor LFA-1 and its ICAM ligands are known to play a role in HIV infection. The presence of these molecules on virions and target cells promotes virus infectivity and has previously been shown to hinder virus neutralization by anti- HIV antibodies. To delineate the effect of these molecules on neutralization of HIV-1, human monoclonal antibodies (MAbs) to V3 and the CD4-binding domain (CD4bd) of gp120 were examined in the presence of anti-LFA-1 MAbs. When either of two anti-LFA-1 MAbs was present, higher levels of virus neutralization were achieved by both anti-V3 and anti-CD4bd MAbs. This effect was observed with primary HIV-1 isolates as well as with a laboratory-adapted strain. However, this activity was seen only when an anti-LFA-1 MAb was combined with anti-gp120 MAbs that exhibited virus-specific neutralizing activities, demonstrating the specificity of both the anti-LFA-1 and anti-gp120 MAbs. Enhanced neutralization by anti-gp120 MAbs was observed if the anti-LFA-1 MAb was present during the initial 24 hr only, if added 24 hr after infection, or if present throughout the culture period. These data suggest that the anti-LFA-1 MAbs could act at different stages of HIV-1 infection, including the initial virus-cell interaction as well as during the amplification and spread of virus from cell to cell. These findings demonstrate the significant role of LFA-1 in HIV-1 infection and have important implications for evaluating the neutralizing activity of anti- HIV antibodies.
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Authors | C E Hioe, J E Hildreth, S Zolla-Pazner |
Journal | AIDS research and human retroviruses
(AIDS Res Hum Retroviruses)
Vol. 15
Issue 6
Pg. 523-31
(Apr 10 1999)
ISSN: 0889-2229 [Print] United States |
PMID | 10221529
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Monoclonal
- HIV Antibodies
- HIV Envelope Protein gp120
- Lymphocyte Function-Associated Antigen-1
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Topics |
- Antibodies, Monoclonal
(immunology)
- Antibody Specificity
- Dose-Response Relationship, Immunologic
- HIV Antibodies
(immunology)
- HIV Envelope Protein gp120
(immunology)
- HIV-1
(immunology)
- Humans
- Lymphocyte Function-Associated Antigen-1
(immunology)
- Neutralization Tests
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