A new protocol to prepare dry plant specimens for electron microscopy and immunocytochemistry.

Abstract	Samples from dry kidney bean cotyledons were fixed in acrolein vapor and embedded in Lowicryl K4M using a modified dehydration and embedding technique. The preservation of morphology as investigated by transmission electron microscopy was excellent showing desiccated cells and organelles in life-like preservation. Postembedding immunogold labeling for kidney bean purple acid phosphatase (KbPAP) showed association of kbPAP with ribosome-rich areas of the cytoplasm.
Authors	M Grote, R Reichelt, R Wiermann
Journal	Micron (Oxford, England : 1993) (Micron) Vol. 30 Issue 1 Pg. 65-70 (Feb 1999) ISSN: 0968-4328 [Print] England
PMID	10216922 (Publication Type: Journal Article)
Chemical References	Glycoproteins Resins, Plant Acrolein purple acid phosphatase Acid Phosphatase
Topics	Acid Phosphatase (analysis) Acrolein Glycoproteins (analysis) Immunohistochemistry (methods) Microscopy, Electron (methods) Resins, Plant Seeds (chemistry, ultrastructure) Tissue Fixation (methods)

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