2,3-Butanedione monoxime cardioplegia: advantages over hyperkalemia in blood-perfused isolated hearts.

Abstract

BACKGROUND:

2,3-Butanedione monoxime (BDM) has been shown to possess cardioprotective properties related to the inhibition of cross-bridge force development, the reduction of myofilament Ca2+ sensitivity, and the attenuation of intracellular Ca2+ transients. This study tested the hypothesis that cardiac arrest achieved with BDM would be as effective as that achieved with St. Thomas' solution (StT).

METHODS:

Isolated rabbit hearts, studied on a blood-perfused Langendorff column, underwent 1 hour of ischemia (37 degrees C) and 30 minutes of reperfusion. Cardioplegia was administered every 20 minutes in the form of (1) Krebs-Henseleit solution only (control), (2) 20 mmol/L of BDM, or (3) StT. Recovery of developed pressure, atrioventricular activation times, and tissue water content were measured.

RESULTS:

Recovery of developed pressure for the control, BDM, and StT groups was 44%+/-3% (p<0.05 versus BDM and StT), 57%+/-5%, and 62%+/-4%, respectively. Atrioventricular activation times were significantly prolonged in the control group (42+/-15 ms, p = 0.042) and the StT group (26+/-9 ms, p = 0.034), but not in the BDM group (14+/-8 ms). Tissue water content after reperfusion was 80%+/-0.4%, 80%+/-0.2%, and 76%+/-1.0% (p<0.05 versus control) in the control, StT, and BDM groups, respectively.

CONCLUSIONS:

2,3-Butanedione monoxime was as effective as StT in protecting the myocardium. Unlike StT, BDM ameliorated myocardial edema and atrioventricular conduction delay after reperfusion.

• Authors: A M Jayawant, E R Stephenson Jr, R J Damiano Jr
• Journal: The Annals of thoracic surgery (Ann Thorac Surg) Vol. 67 Issue 3 Pg. 618-23 (Mar 1999) ISSN: 0003-4975 [Print] Netherlands
• PMID: 10215198 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Bicarbonates
• Cardioplegic Solutions
• Krebs-Henseleit solution
• St. Thomas' Hospital cardioplegic solution
• Tromethamine
• diacetylmonoxime
• Sodium Chloride
• Potassium Chloride
• Magnesium
• Glucose
• Diacetyl
• Calcium Chloride
• Potassium

Topics

• Animals
• Bicarbonates
• Calcium Chloride
• Cardioplegic Solutions
• Coronary Circulation
• Diacetyl (analogs & derivatives)
• Glucose
• Heart (physiopathology)
• Heart Arrest, Induced
• In Vitro Techniques
• Magnesium
• Myocardial Reperfusion
• Myocardium (metabolism)
• Potassium (blood)
• Potassium Chloride
• Rabbits
• Sodium Chloride
• Tromethamine