DNA-dependent protein kinase content and activity in lung carcinoma cell lines: correlation with intrinsic radiosensitivity.

Abstract

Intrinsic radiosensitivity and rejoining of radiation-induced DNA double-strand breaks (DNA-dsb) were analysed in five lung carcinoma cell lines: U-1285, U-1906, H-69, H-82 and U-1810. RS correlated with both the initial phase of DNA-dsb rejoining, at 15 min (r2 = 0.818) and the late phase, at 120 min postirradiation (r2 = 0.774), the most sensitive cell line (U-1285) showing least dsb rejoining and the most resistant (U-1810) showing most dsb rejoining of all five cell lines studied. As DNA-PK has been recognised as an important molecular component involved in DNA-dsb repair, we analysed content and activity of this kinase. We found that DNA-PK content and activity correlated with RS (r2 = 0.941 and r2 = 0.944, respectively). The lowest DNA-dependent content/activity was found in the most radiosensitive cells, U-1285 and H-69, whilst the highest content/activity was found in the most radioresistant cells U-1810. These results suggest a correlation between RS and DNA-PK content/activity in lung carcinoma cell lines.

• Authors: F Sirzén, A Nilsson, B Zhivotovsky, R Lewensohn
• Journal: European journal of cancer (Oxford, England : 1990) (Eur J Cancer) Vol. 35 Issue 1 Pg. 111-6 (Jan 1999) ISSN: 0959-8049 [Print] England
• PMID: 10211098 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• DNA-Binding Proteins
• Nuclear Proteins
• DNA-Activated Protein Kinase
• PRKDC protein, human
• Protein Serine-Threonine Kinases

Topics

• Blotting, Western
• Carcinoma, Small Cell (enzymology, genetics)
• DNA Repair
• DNA, Neoplasm (metabolism, radiation effects)
• DNA-Activated Protein Kinase
• DNA-Binding Proteins
• Humans
• Lung Neoplasms (enzymology, genetics)
• Nuclear Proteins
• Protein Serine-Threonine Kinases (metabolism)
• Radiation Tolerance
• Tumor Cells, Cultured (radiation effects)