Limbic seizures increase pronociceptin mRNA levels in the thalamic reticular nucleus.

Abstract

We studied pronociceptin gene expression following limbic seizures. Northern blot analysis revealed increased pronociceptin mRNA levels in the thalamus (but not in the hippocampus) 3-24 h after kainate administration, with maximal effect (2-fold increase over basal levels) reached at 6 h. No variation in pronociceptin mRNA levels was observed 1-6 h after a stimulus-evoked kindled seizure. Carrageenan failed to affect pronociceptin gene expression in the thalamus, indicating that pain and/or acute stress do not account for kainate effects. In situ hybridization revealed that kainate evokes a dramatic (4-fold) increase in pronociceptin mRNA levels over the thalamic reticular nucleus. Kindled seizures evoked only a small, non-significant increase in pronociceptin gene expression over the dentate gyrus of the hippocampus.

• Authors: G Bregola, S Candeletti, P Romualdi, M Simonato
• Journal: Neuroreport (Neuroreport) Vol. 10 Issue 3 Pg. 541-6 (Feb 25 1999) ISSN: 0959-4965 [Print] England
• PMID: 10208586 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protein Precursors
• RNA, Messenger
• Receptors, Opioid
• pronociceptin
• Kainic Acid

Topics

• Animals
• Blotting, Northern
• In Situ Hybridization
• Kainic Acid
• Kindling, Neurologic (physiology)
• Limbic System (physiopathology)
• Male
• Protein Precursors (genetics)
• RNA, Messenger (metabolism)
• Rats
• Rats, Sprague-Dawley
• Receptors, Opioid (genetics)
• Seizures (chemically induced, etiology, metabolism, physiopathology)
• Thalamic Nuclei (metabolism)