Failure of endotoxin-free superoxide dismutase to reduce some paw edemas and adjuvant arthritis in rats.
|
| Abstract | OBJECTIVE AND DESIGN: To elucidate whether superoxide anion plays a major role in inflammation models in rats. Endotoxin-free CuZn superoxide (SOD) was used in the present study, because we had shown previously that even a trace of endotoxin contamination of SOD would carry anti-inflammatory effects. MATERIAL AND TREATMENT: Male rats were treated with saline (n=8-10), recombinant human SOD (n=8-10), and dexamethasone or indomethacin (n=8-10). METHODS: Foot volume was determined by mercury displacement. RESULTS: Two administrations of SOD (5 and 50 mg/kg), 2 h and just before irritant injection, had no effect on rat paw edema induced by phorbol, zymosan or carrageenan. Two administrations of dexamethasone (1 mg/kg) or indomethacin (3 mg/kg), 2 h and just before irritation, inhibited paw edema significantly (p<0.05). Administration of SOD (10 mg/kg) once daily for 3 weeks from the day of mycobacterium injection had no effect on injected paw swelling and non-injected paw swelling 7, 14, 21 days after irritation. Indomethacin (2 mg/kg) once daily for 3 weeks inhibited this arthritis significantly (p < 0.05). CONCLUSIONS:
Superoxide anion may not play a major role in the genesis of inflammation in these rat inflammation models, and endotoxin-like contaminants may have contributed to some protective effects of SOD administration in previous studies.
|
|---|---|
| Authors | M Iida, K Saito |
| Journal | Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 48 Issue 2 Pg. 63-6 (Feb 1999) ISSN: 1023-3830 [Print] Switzerland |
| PMID | 10202990 (Publication Type: Journal Article) |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!