Abstract | BACKGROUND: METHODS: Isolated rat livers underwent 30 min of perfusion, 2.5 h of ischemia, and 3 h of reperfusion. The temperature was maintained at 34 degrees C (mild hypothermia, n = 5) or 38 degrees C (normothermia, n = 6) for all three periods by perfusion of a modified Krebs Henseleit solution, air surface cooling, or both. A third group of livers was normothermic before and during ischemia and mildly hypothermic during reperfusion (reperfusion hypothermia, n = 6). Control livers had 3 h of perfusion at normothermia. Chemiluminescence (a measure of the generation of reactive oxygen species) and hepatic vascular resistance were monitored simultaneously to evaluate the effect of temperature on the formation of reactive oxygen species and the development of no reflow. Also measured were thiobarbituric acid reactive species and lactate dehydrogenase, as indicators of oxidative stress and cell injury. RESULTS: CONCLUSION: The prevention of hepatic vascular injury by suppression of oxidative stress may be an important protective mechanism of mild hypothermia.
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Authors | H A Zar, K Tanigawa, Y M Kim, J R Lancaster Jr |
Journal | Anesthesiology
(Anesthesiology)
Vol. 90
Issue 4
Pg. 1103-11
(Apr 1999)
ISSN: 0003-3022 [Print] United States |
PMID | 10201683
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Reactive Oxygen Species
- L-Lactate Dehydrogenase
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Topics |
- Animals
- Hyperthermia, Induced
- Ischemia
(physiopathology)
- L-Lactate Dehydrogenase
(metabolism)
- Lipid Peroxidation
- Liver
(blood supply)
- Luminescent Measurements
- Male
- Oxygen Consumption
- Perfusion
- Rats
- Rats, Sprague-Dawley
- Reactive Oxygen Species
(metabolism)
- Vascular Resistance
- Vasoconstriction
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