Abstract |
c-Fos, a component of the dimeric transcription factor AP-1, is necessary for osteoclast formation. To determine whether c-Fos can substitute for any or all of the stimuli needed for osteoclast induction, we infected osteoclast precursors with retroviral vectors expressing c-Fos or the Fos-related protein, Fra-1. The infected cells were incubated with or without osteoclast-inductive stimuli. Osteoclast formation from retroviral-infected precursors remained completely dependent on osteoclast-inductive stromal cells. Unexpectedly, infection of bipotential osteoclast-macrophage precursor cell lines with retroviruses expressing Fra-1 but not c-Fos caused a 10-100-fold increase in the number of precursors that developed calcitonin receptors associated with an increase in bone resorption. These observations suggest that, in the precursor cell lines, Fra-1 is a limiting factor for full responsiveness to the osteoclast-inductive environment. Fra-1 is therefore likely to play a role in osteoclast differentiation which is distinct from that of c-Fos.
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Authors | J M Owens, K Matsuo, G C Nicholson, E F Wagner, T J Chambers |
Journal | Journal of cellular physiology
(J Cell Physiol)
Vol. 179
Issue 2
Pg. 170-8
(May 1999)
ISSN: 0021-9541 [Print] United States |
PMID | 10199556
(Publication Type: Journal Article)
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Chemical References |
- Proto-Oncogene Proteins c-fos
- RNA, Messenger
- Receptors, Calcitonin
- fos-related antigen 1
- Macrophage Colony-Stimulating Factor
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Topics |
- Autoradiography
- Bone Resorption
(metabolism)
- Cell Differentiation
(genetics)
- Cell Line
- Gene Expression Regulation
(genetics)
- Macrophage Colony-Stimulating Factor
(metabolism)
- Macrophages
(metabolism)
- Osteoclasts
(metabolism)
- Phenotype
- Proto-Oncogene Proteins c-fos
(genetics, metabolism)
- RNA, Messenger
(analysis)
- Receptors, Calcitonin
(metabolism)
- Retroviridae
(genetics)
- Transfection
(genetics)
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