Inhibitors of tryptase for the treatment of mast cell-mediated diseases.

Abstract

Human tryptase is a structurally unique and mast cell specific trypsin-like serine protease. Recent biological and immunological investigations have implicated tryptase as a mediator in the pathology of numerous allergic and inflammatory conditions including rhinitis, conjunctivitis, and most notably asthma. A growing body of data further implicates tryptase in certain gastrointestinal, dermatological, and cardiovascular disorders as well. The recent availability of potent, and selective tryptase inhibitors, though, has facilitated the validation of this protease as an important therapeutic target as well. Herein, we describe the design and potency of four classes of selective tryptase inhibitors, of which the first three types are synthetic and the fourth is natural in origin: 1) peptidic inhibitors (e.g., APC-366), 2) dibasic inhibitors (i.e., pentamidine-like), 3) Zn(2+)-mediated inhibitors (i.e., BABIM-like), and 4) heparin antagonists (e.g., lactoferrin). These inhibitors have been tested in the airways and skin of allergic sheep. Aerosol administration of tryptase inhibitors from each structural class 30 minutes before, and 4 hours and 24 hours after allergen challenge, abolishes late phase bronchoconstriction and airway hyperresponsiveness in a dose-dependent manner. Moreover, intradermal injection of APC-366 blocks the cutaneous response to antigen. These studies provide the essential proof-of-concept for the further pursuit of tryptase inhibitors for the treatment of asthma, and perhaps other allergic diseases. Results from clinical studies with the first generation tryptase inhibitor APC-366, currently in phase II trials for the treatment of asthma, provide additional support for a pathological role for tryptase in this disease. Notable advances in the area of tryptase inhibitor design at Axys Pharmaceuticals, Inc. include a novel, zinc-mediated, serine protease inhibitor technology (described herein), and the discovery of a unique class of extremely potent and selective dibasic tryptase inhibitors. Independently, an X-ray crystal structure of active tryptase tetramer complexed with 4-amidinophenyl pyruvic acid has been reported. It is anticipated that these discoveries will further accelerate the design of structurally novel tryptase inhibitors as well as the development of new drugs for the treatment of mast cell tryptase-mediated disorders.

• Authors: K D Rice, R D Tanaka, B A Katz, R P Numerof, W R Moore
• Journal: Current pharmaceutical design (Curr Pharm Des) Vol. 4 Issue 5 Pg. 381-96 (Oct 1998) ISSN: 1381-6128 [Print] United Arab Emirates
• PMID: 10197050 (Publication Type: Journal Article, Review)

Chemical References

• Inflammation Mediators
• Serine Proteinase Inhibitors
• Serine Endopeptidases
• chymase 2
• Chymases
• Tryptases

Topics

• Animals
• Chymases
• Drug Design
• Humans
• Immune System Diseases (drug therapy)
• Inflammation (drug therapy)
• Inflammation Mediators (physiology)
• Mast Cells (drug effects, enzymology)
• Models, Molecular
• Serine Endopeptidases (drug effects)
• Serine Proteinase Inhibitors (classification, pharmacology, therapeutic use)
• Tryptases