Abstract | BACKGROUND AND OBJECTIVES: STUDY DESIGN: The cytotoxicity of nonoxynol-9 alone or incorporated into the gel was investigated in human cervical and colon epithelial cells and after daily intravaginal application for 2 weeks in rabbits. RESULTS: In vitro experiments showed that nonoxynol-9 was highly toxic to human cervical and colon epithelial cells in a dose-dependent manner. However, the incorporation of the spermicide into the gel markedly reduced its toxicity under the same experimental conditions. In vivo studies showed that in animals treated with nonoxynol-9, the spermicide was very toxic to the vaginal and cervical mucosa as evidenced by the presence of bleeding, irritation, epithelial disruption, necrosis, the accumulation of leukocytes in the submucosa, and the loss of integrity of the epithelial cells. Of prime importance, the incorporation of nonoxynol-9 into the gel markedly reduced the toxicity of this potent spermicide/ microbicide. CONCLUSION: The gel formulation could be used as an interesting approach to eliminate the toxicity of potent spermicides/ microbicides such as nonoxynol-9.
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Authors | N Gagné, H Cormier, R F Omar, A Désormeaux, P Gourde, M J Tremblay, J Juhász, D Beauchamp, J E Rioux, M G Bergeron |
Journal | Sexually transmitted diseases
(Sex Transm Dis)
Vol. 26
Issue 3
Pg. 177-83
(Mar 1999)
ISSN: 0148-5717 [Print] United States |
PMID | 10100776
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Gels
- Polymers
- Polypropylenes
- Protective Agents
- Nonoxynol
- Polyethylene Glycols
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Topics |
- Animals
- Diffusion
- Drug Interactions
- Female
- Gels
- Mucous Membrane
(drug effects)
- Nonoxynol
(toxicity)
- Polyethylene Glycols
- Polymers
- Polypropylenes
- Protective Agents
- Rabbits
- Tumor Cells, Cultured
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