Regulation of c-fos gene transcription and myeloid cell differentiation by acute myeloid leukemia 1 and acute myeloid leukemia-MTG8, a chimeric leukemogenic derivative of acute myeloid leukemia 1.

Abstract

Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Jurkat T-cells, but not by that of the short form of acute myeloid leukemia 1-MTG8, a chimeric acute myeloid leukemia 1 protein. In 32Dcl3 myeloid cells, stable overexpression of acute myeloid leukemia 1-MTG8 blocked the c-fos gene transcription and cell differentiation, but that of acute myeloid leukemia did not. These data suggest that acute myeloid leukemia 1 and acute myeloid leukemia 1-MTG8 reciprocally regulate the myeloid cell differentiation, possibly by the way of regulating c-fos gene transcription.

• Authors: E S Hwang, J H Hong, S C Bae, Y Ito, S K Lee
• Journal: FEBS letters (FEBS Lett) Vol. 446 Issue 1 Pg. 86-90 (Mar 05 1999) ISSN: 0014-5793 [Print] England
• PMID: 10100620 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Core Binding Factor Alpha 2 Subunit
• DNA-Binding Proteins
• Neoplasm Proteins
• Proto-Oncogene Proteins
• RUNX1 Translocation Partner 1 Protein
• RUNX1 protein, human
• RUNX1T1 protein, human
• Recombinant Fusion Proteins
• Transcription Factors

Topics

• Cell Differentiation (genetics)
• Core Binding Factor Alpha 2 Subunit
• DNA-Binding Proteins (genetics)
• Gene Expression Regulation, Neoplastic
• Genes, fos
• Humans
• Jurkat Cells
• Leukopoiesis (genetics)
• Neoplasm Proteins (genetics)
• Proto-Oncogene Proteins
• RUNX1 Translocation Partner 1 Protein
• Recombinant Fusion Proteins (genetics)
• Transcription Factors (genetics)
• Transcription, Genetic