Abstract |
Transient expression of the differentiation and tumor cell surface antigen gp130(RB13-6) characterizes a subset of rat glial progenitor cells susceptible to ethylnitrosourea-induced neurooncogenesis. gp130(RB13-6) is as a member of an emerging protein family of ecto- phosphodiesterases/ nucleotide pyrophosphatases that includes PC-1 and the tumor cell motility factor autotaxin. We have investigated the potential role of gp130(RB13-6) in glial differentiation by transfection of three cell lines of different origin that do not express endogenous gp130(RB13-6) (NIH-3T3 mouse fibroblasts; C6 and BT7Ca rat glioma cells) with the cDNA encoding gp130(RB13-6). The effect of gp130(RB13-6) expression was analyzed in terms of overall cell morphology, the expression of glial cell-specific marker proteins, and invasiveness. Transfectant sublines, consisting of 100% gp130(RB13-6)-positive cells, exhibited an altered, bipolar morphology. Fascicular aggregates of fibroblastoid cells subsequently developed into mesh-like patterns. Contrary to the parental NIH-3T3 and BT7Ca cells, the transfectant cells invaded into collagen type I. As shown by immunofluorescence staining of the transfectant sublines as well as of primary cultures composed of gp130(RB13-6)-positive and -negative cells, expression of gp130(RB13-6) induced coexpression of proteins typical for glial cells and their precursors, i.e., glial fibrillary acidic protein, the low affinity nerve growth factor receptor, and the neural proteins Thy-1, Ran-2, and S-100. In accordance with its expression in the immature rat nervous system, gp130(RB13-6) may thus have a significant role in the glial differentiation program and its subversion in neurooncogenesis.
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Authors | H Deissler, S Blass-Kampmann, E Bruyneel, M Mareel, M F Rajewsky |
Journal | FASEB journal : official publication of the Federation of American Societies for Experimental Biology
(FASEB J)
Vol. 13
Issue 6
Pg. 657-66
(Apr 1999)
ISSN: 0892-6638 [Print] United States |
PMID | 10094926
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Differentiation
- Antigens, Neoplasm
- Lamp1 protein, rat
- Lysosome-Associated Membrane Glycoproteins
- Membrane Glycoproteins
- Recombinant Proteins
- Collagen
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Topics |
- Animals
- Antigens, Differentiation
(biosynthesis)
- Antigens, Neoplasm
(biosynthesis, genetics)
- Astrocytes
(metabolism, pathology)
- Cell Differentiation
- Collagen
(pharmacology)
- Fibroblasts
(metabolism, pathology)
- Glioma
(metabolism, pathology)
- Lysosome-Associated Membrane Glycoproteins
- Membrane Glycoproteins
(biosynthesis, genetics)
- Neoplasm Invasiveness
- Neuroglia
(metabolism, pathology)
- Rats
- Recombinant Proteins
(biosynthesis)
- Schwann Cells
(metabolism, pathology)
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