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Neural cell surface differentiation antigen gp130(RB13-6) induces fibroblasts and glioma cells to express astroglial proteins and invasive properties.

Abstract
Transient expression of the differentiation and tumor cell surface antigen gp130(RB13-6) characterizes a subset of rat glial progenitor cells susceptible to ethylnitrosourea-induced neurooncogenesis. gp130(RB13-6) is as a member of an emerging protein family of ecto-phosphodiesterases/nucleotide pyrophosphatases that includes PC-1 and the tumor cell motility factor autotaxin. We have investigated the potential role of gp130(RB13-6) in glial differentiation by transfection of three cell lines of different origin that do not express endogenous gp130(RB13-6) (NIH-3T3 mouse fibroblasts; C6 and BT7Ca rat glioma cells) with the cDNA encoding gp130(RB13-6). The effect of gp130(RB13-6) expression was analyzed in terms of overall cell morphology, the expression of glial cell-specific marker proteins, and invasiveness. Transfectant sublines, consisting of 100% gp130(RB13-6)-positive cells, exhibited an altered, bipolar morphology. Fascicular aggregates of fibroblastoid cells subsequently developed into mesh-like patterns. Contrary to the parental NIH-3T3 and BT7Ca cells, the transfectant cells invaded into collagen type I. As shown by immunofluorescence staining of the transfectant sublines as well as of primary cultures composed of gp130(RB13-6)-positive and -negative cells, expression of gp130(RB13-6) induced coexpression of proteins typical for glial cells and their precursors, i.e., glial fibrillary acidic protein, the low affinity nerve growth factor receptor, and the neural proteins Thy-1, Ran-2, and S-100. In accordance with its expression in the immature rat nervous system, gp130(RB13-6) may thus have a significant role in the glial differentiation program and its subversion in neurooncogenesis.
AuthorsH Deissler, S Blass-Kampmann, E Bruyneel, M Mareel, M F Rajewsky
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology (FASEB J) Vol. 13 Issue 6 Pg. 657-66 (Apr 1999) ISSN: 0892-6638 [Print] United States
PMID10094926 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Differentiation
  • Antigens, Neoplasm
  • Lamp1 protein, rat
  • Lysosome-Associated Membrane Glycoproteins
  • Membrane Glycoproteins
  • Recombinant Proteins
  • Collagen
Topics
  • Animals
  • Antigens, Differentiation (biosynthesis)
  • Antigens, Neoplasm (biosynthesis, genetics)
  • Astrocytes (metabolism, pathology)
  • Cell Differentiation
  • Collagen (pharmacology)
  • Fibroblasts (metabolism, pathology)
  • Glioma (metabolism, pathology)
  • Lysosome-Associated Membrane Glycoproteins
  • Membrane Glycoproteins (biosynthesis, genetics)
  • Neoplasm Invasiveness
  • Neuroglia (metabolism, pathology)
  • Rats
  • Recombinant Proteins (biosynthesis)
  • Schwann Cells (metabolism, pathology)

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