Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M.

Abstract

The responses of the trichloroethylene-degrading bacterium Methylocystis sp. M to six different water-pollutants, carbon starvation, and temperature-shock (heat and cold) were examined using 2-dimensional gel electrophoresis. Twenty-eight polypeptides were induced, and these stress-induced proteins were classified into three groups. Some of the chemically induced proteins were the same as those induced by carbon starvation and temperature-shock. Two of the polypeptides were induced by trichloroethylene. Trichloroethylene-stress protein synthesis required 1-2 h at a concentration of trichloroethylene that had no effect on growth. Furthermore, 25 stress-enhanced polypeptides were observed, and one of these was enhanced by trichloroethylene. Based on these results, we discuss applications of chemical-stress induction of proteins to establish effective bioremediation and bioassay by methanotrophs.

• Authors: H Uchiyama, Y Shinohara, N Tomioka, I Kusakabe
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 170 Issue 1 Pg. 125-30 (Jan 01 1999) ISSN: 0378-1097 [Print] England
• PMID: 10094607 (Publication Type: Journal Article)

Chemical References

• Bacterial Proteins
• Heat-Shock Proteins
• Hydrocarbons, Chlorinated
• Water Pollutants, Chemical
• Trichloroethylene
• Carbon

Topics

• Bacteria (drug effects, metabolism)
• Bacterial Proteins (biosynthesis)
• Biodegradation, Environmental
• Carbon (metabolism)
• Electrophoresis, Gel, Two-Dimensional
• Heat-Shock Proteins (biosynthesis)
• Hydrocarbons, Chlorinated (pharmacology)
• Kinetics
• Temperature
• Trichloroethylene (metabolism, pharmacology)
• Water Pollutants, Chemical (pharmacology)