Evaluation of a hybrid artificial liver using a polyurethane foam packed-Bed culture system in dogs.

Abstract

BACKGROUND AND AIMS:

We developed a polyurethane foam packed-bed culture system of hepatocyte spheroids as a hybrid artificial liver (PUF-HAL), which was effective for recovery from liver failure in rat experiments. In this report, the design of a scaled-up PUF-HAL for dogs is described and evaluated using a dog acute liver failure model.

METHODS:

Warm ischemic liver failure was induced with a portocaval shunt in each dog. The dogs were divided into two groups: (1) a control group (N = 4), in which each dog was attached to a PUF-HAL without hepatocytes for 9 h, and (2) a HAL group (N = 5), in which each dog was attached to a PUF-HAL with hepatocytes. Blood pressure, blood ammonia, blood glucose, serum creatinine, and other parameters related to liver function were compared between the two groups.

RESULTS:

In the HAL group, blood ammonia and serum creatinine levels were significantly lower, and blood pressure and blood glucose levels significantly higher, than those in the control group.

CONCLUSIONS:

The scaled-up PUF-HAL developed for large animals is useful as a liver support system in the dog acute liver failure model.

• Authors: T Gion, M Shimada, K Shirabe, K Nakazawa, H Ijima, T Matsushita, K Funatsu, K Sugimachi
• Journal: The Journal of surgical research (J Surg Res) Vol. 82 Issue 2 Pg. 131-6 (Apr 1999) ISSN: 0022-4804 [Print] United States
• PMID: 10090820 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 1999 Academic Press.

Chemical References

• Blood Glucose
• Polyurethanes
• Ammonia
• Creatinine

Topics

• Ammonia (blood)
• Animals
• Blood Glucose (analysis)
• Blood Pressure
• Cell Culture Techniques (methods)
• Creatinine (blood)
• Dogs
• Extracorporeal Circulation
• Female
• Liver (cytology)
• Liver Failure (blood, physiopathology, therapy)
• Liver, Artificial
• Male
• Polyurethanes (chemistry)
• Porosity
• Spheroids, Cellular