Hypertonic saline dextran attenuates leukocyte accumulation in the liver after hemorrhagic shock and resuscitation.

Abstract

BACKGROUND:

Hemorrhagic shock and resuscitation triggers a global ischemia/reperfusion phenomenon, in which activated leukocytes are considered strong contributors to the ensuing tissue damage.

METHODS:

The aim of the study was to investigate the effects of hypertonic saline dextran (HSD) on the early leukocyte/endothelial interactions (intravital fluorescence microscopy) in a rat model of hemorrhagic shock (1 hour at mean arterial pressure of 40 mm Hg). The resuscitation was performed with lactated Ringer's solution (RL, four times shed blood/20 minutes, n = 6), 6% dextran 60 (DEX, 100% shed blood/5 minutes, n = 8), and 7.2% NaCl/10% dextran 60 (HSD, 10% shed blood/2 minutes, n = 8).

RESULTS:

After 1 hour of resuscitation, shock-induced stasis/adherence of leukocytes was further enhanced with RL (sinusoids 17.6+/-6.9%; venules 33.9+/-8.5%), whereas DEX and HSD attenuated leukocyte stagnation in sinusoids (DEX -7.4+/-6,1%; HSD -14.7+/-2.9%, p<0.01 vs. RL) and leukocyte adherence in postsinusoidal venules (DEX -12.2+/-8.6%, p<0.05 vs. RL; HSD -27+/-7.4%, p<0.01 vs. RL).

CONCLUSION:

HSD reduced significantly the number of leukocytes accumulated in the liver after resuscitation of hemorrhagic shock, probably due to a combination of mechanisms of both components.

• Authors: C O Corso, S Okamoto, D Rüttinger, K Messmer
• Journal: The Journal of trauma (J Trauma) Vol. 46 Issue 3 Pg. 417-23 (Mar 1999) ISSN: 0022-5282 [Print] United States
• PMID: 10088843 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dextrans
• Isotonic Solutions
• Ringer's Lactate
• hypertonic saline-dextran solution
• Sodium Chloride

Topics

• Animals
• Dextrans (therapeutic use)
• Disease Models, Animal
• Drug Evaluation, Preclinical
• Endothelium (immunology)
• Humans
• Isotonic Solutions (therapeutic use)
• Laser-Doppler Flowmetry
• Leukocytes (drug effects, immunology)
• Liver (immunology)
• Male
• Microscopy, Fluorescence
• Rats
• Rats, Wistar
• Reperfusion Injury (etiology, immunology, therapy)
• Resuscitation (adverse effects, methods)
• Ringer's Lactate
• Shock, Hemorrhagic (complications, therapy)
• Sodium Chloride (therapeutic use)
• Time Factors