Abstract | OBJECTIVE: METHODS: RESULTS: Reactivity of the 846 epitope was increased in all study groups compared with the reference group, and was highest in patients with primary OA. The median levels (in microg fetal aggrecan equivalents/ml) of the epitope were 0.28 (range 0.24-0.47) in the reference group, 0.48 (range 0.26-1.32) in PPA/ pseudogout, 0.61 (range 0.12-2.87) in ACL rupture, 0.53 (range 0.22-3.02) in meniscus lesion, and 0.68 (range 0.31-4.31) in primary OA. The 846 epitope reactivity per microg aggrecan fragments in the joint fluid was higher in late-stage OA than in early-stage OA. Epitope 846 reactivity correlated positively with several markers of matrix turnover, particularly with COMP (r(s) = 0.421) and CPII (r(s) = 0.307). CONCLUSION: The observed differences in 846 epitope reactivity in synovial fluid, and its concentration in relation to aggrecan and other markers of matrix turnover, were consistent with marked ongoing changes in aggrecan turnover after joint injury and in the development of OA. OA is thus a disease characterized by dynamic changes in tissue macromolecule turnover, which is reflected by measurable changes in aggrecan epitopes in the synovial fluid.
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Authors | L S Lohmander, M Ionescu, H Jugessur, A R Poole |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 42
Issue 3
Pg. 534-44
(Mar 1999)
ISSN: 0004-3591 [Print] United States |
PMID | 10088777
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Aggrecans
- Autoantibodies
- Biomarkers
- Epitopes
- Extracellular Matrix Proteins
- Lectins, C-Type
- Proteoglycans
- Alcian Blue
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Topics |
- Aggrecans
- Alcian Blue
- Autoantibodies
(analysis)
- Biomarkers
- Cartilage, Articular
(immunology, metabolism)
- Enzyme-Linked Immunosorbent Assay
- Epitopes
(metabolism)
- Extracellular Matrix Proteins
- Humans
- Knee Injuries
(immunology, metabolism)
- Lectins, C-Type
- Osteoarthritis
(metabolism)
- Proteoglycans
(immunology, metabolism)
- Regression Analysis
- Synovial Fluid
(metabolism)
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