Abstract |
The protooncogenes c-myc and c-myb are erythropoietin (Epo)-regulated early response genes. They appear to play distinct roles in the growth- and differentiation-inducing signals of the hormone. Using a subtractive hybridization strategy, we have identified the murine homolog of the TAX response element- binding protein TAXREB107 as an Epo early response gene in Rauscher murine erythroleukemia cells. This was confirmed by Northern blot analyses, which showed a fourfold increase in TAXREB107 mRNA after 1 hr of erythropoietin treatment. After 3 hr the transcript had decreased to approximately twofold above control levels. Inhibition of this induction with antisense oligodeoxynucleotides increased Epo-induced hemoglobinization 2.5-fold. The results implicate TAXREB107 in erythropoiesis and support the hypothesis that the TAXREB proteins have functions outside the context of HTLV-I infection.
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Authors | R Li, H Madden, A J Sytkowski |
Journal | AIDS research and human retroviruses
(AIDS Res Hum Retroviruses)
Vol. 15
Issue 4
Pg. 375-9
(Mar 01 1999)
ISSN: 0889-2229 [Print] United States |
PMID | 10082121
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA-Binding Proteins
- tax-responsive-element-binding protein 107, mouse
- Erythropoietin
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Topics |
- Animals
- DNA-Binding Proteins
(genetics)
- Erythropoietin
(metabolism, pharmacology)
- Humans
- Mice
- Tumor Cells, Cultured
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