A serosurvey of Borna disease virus infection in wild rats by a capture ELISA.

Abstract

For a serological diagnostic test for Borna disease (BD), we developed a capture ELISA with specificity and sensitivity based on detection of antibodies against BD virus (BDV) p40 protein. Using our capture ELISA system, the antibody response of rats inoculated intracerebrally with BDV at 4 weeks after birth showed a sharp increase from 1 to 4 weeks postinoculation (p.i.) and a steady level after 5 weeks p.i. To investigate prevalence of BDV infection among wild rats, we examined sera of Rattus norvegicus in Kami-iso town, Oshima district, Hokkaido, suggesting that rats in this area had not been infected by BDV.

• Authors: K Tsujimura, T Mizutani, H Kariwa, K Yoshimatsu, M Ogino, Y Morii, H Inagaki, J Arikawa, I Takashima
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 61 Issue 2 Pg. 113-7 (Feb 1999) ISSN: 0916-7250 [Print] Japan
• PMID: 10081747 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Viral Proteins
• p40 protein, Borna disease virus

Topics

• Animals
• Antibodies, Monoclonal
• Antibodies, Viral (blood)
• Blotting, Western (veterinary)
• Borna Disease (epidemiology)
• Borna disease virus (immunology)
• Cell Line
• Dogs
• Enzyme-Linked Immunosorbent Assay (methods, veterinary)
• Japan (epidemiology)
• Mice
• Mice, Inbred BALB C
• Rats
• Rodent Diseases (epidemiology)
• Viral Proteins (immunology)