Abstract |
Zinc acexamate (NAS-501), an anti- ulcer agent, has been reported to prevent various acute experimental gastric mucosal lesions and duodenal ulcers in rats. In order to clarify the mechanisms by which NAS-501 exhibits the anti- ulcer effects, we investigated the anti-oxidative effects of NAS-501 in vitro and in vivo. NAS-501 significantly reduced the superoxide radical-dependent chemiluminescence, generated by hypoxanthine-xanthine oxidase, rat neutrophils and guinea-pig macrophages in vitro. These in vitro effects were also confirmed by electron spin resonance using a 5, 5-dimethyl-1-pyrroline-N-oxide spin-trapping method. In addition, NAS-501 significantly inhibited lipid peroxidation induced by increasing concentrations of Fe2+/ascorbate in rat gastric mucosal homogenate in vitro. Oral administration of NAS-501 (30 mg/kg) significantly inhibited production of thiobarbituric acid-reactive substance in rat gastric mucosa following per os instillation of 60% ethanol in 150 mmol/l HCl in vivo. These results suggest that NAS-501 exhibits the preventive effect from acute gastric mucosal lesions by the anti-oxidative activity.
|
Authors | Y Tsutsui, Y Nakamura, S Yamaguchi, N Kawanaka, M Sato |
Journal | Pharmacology
(Pharmacology)
Vol. 58
Issue 4
Pg. 209-19
(Apr 1999)
ISSN: 0031-7012 [Print] Switzerland |
PMID | 10077740
(Publication Type: Journal Article)
|
Chemical References |
- Aminocaproates
- Anti-Ulcer Agents
- Antioxidants
- Pyrazines
- Thiobarbituric Acid Reactive Substances
- 2-methyl-6-phenyl-3,7-dihydroimidazo(1,2-a)pyrazin-3-one
- Superoxides
- Hypoxanthine
- 6-acetylaminocaproic acid
- Xanthine Oxidase
- Aminocaproic Acid
|
Topics |
- Aminocaproates
- Aminocaproic Acid
(pharmacology)
- Animals
- Anti-Ulcer Agents
(pharmacology)
- Antioxidants
(pharmacology)
- Electron Spin Resonance Spectroscopy
- Gastric Mucosa
(drug effects, metabolism, pathology)
- Guinea Pigs
- Hypoxanthine
(metabolism)
- Lipid Peroxidation
(drug effects)
- Luminescent Measurements
- Macrophages
(cytology, drug effects, metabolism)
- Male
- Neutrophils
(cytology, drug effects, metabolism)
- Pyrazines
- Rats
- Rats, Sprague-Dawley
- Superoxides
(metabolism)
- Thiobarbituric Acid Reactive Substances
(metabolism)
- Xanthine Oxidase
(metabolism)
|