Inhibition of lung metastasis by adoptive immunotherapy using Iscador.

Abstract

Adoptive immunotherapy using spleen cells activated with Iscador was found to inhibit tumour growth significantly (P<0.001). Metastatic B16F10 tumour bearing animals treated with a single dose of spleen cells activated with Iscador (in vitro) showed 100% inhibition of tumour nodule formation on 21st day. Single injection of splenocytes isolated from mice treated with Iscador inhibited the tumour nodule formation by 93.8%. Animals treated with in vivo and in vitro activated spleen cells along with Iscador had an increase in life span of 119% and 81% respectively. Treatment of animals with low dose of Iscador after adoptive immunotherapy further augmented the life span. Animals which underwent adoptive immunotherapy showed significantly reduced lung collagen hydroxyproline (9.8 microg/mg protein) and serum sialic acid (24.61 micro/ml serum) levels compared to control tumour bearing animals with increased levels of lung hydroxyproline (26.95 microg/mg protein) and serum sialic acid levels (151.3 microg/ml serum). Serum gamma -glutamyl transpeptidase levels were found to be significantly reduced in the group of animals treated with Iscador and Iscador activated splenocytes (16.6+/-2.3 nmol P-nitroaniline released /ml serum) Group of animals treated with Iscador activated splenocytes alone also showed significantly reduced serum gamma - glutamyl transpeptidase levels (17.3+/-10 nmol P-nitroaniline released /ml serum) compared to control tumour bearing animals (91+/-12 nmol P-nitroaniline released /ml serum).

• Authors: S Antony, R Kuttan, G Kuttan
• Journal: Immunological investigations (Immunol Invest) Vol. 28 Issue 1 Pg. 1-8 (Jan 1999) ISSN: 0882-0139 [Print] England
• PMID: 10073677 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Plant Extracts
• Plant Proteins
• Collagen
• viscum album peptide
• gamma-Glutamyltransferase
• N-Acetylneuraminic Acid
• Hydroxyproline

Topics

• Animals
• Antineoplastic Agents, Phytogenic (therapeutic use)
• Collagen (chemistry)
• Hydroxyproline (analysis)
• Immunotherapy, Adoptive
• Lung (chemistry)
• Lung Neoplasms (immunology, secondary, therapy)
• Melanoma, Experimental (secondary, therapy)
• Mice
• Mice, Inbred C57BL
• N-Acetylneuraminic Acid (blood)
• Plant Extracts (therapeutic use)
• Plant Proteins
• Spleen (cytology, immunology)
• gamma-Glutamyltransferase (metabolism)