Gonadorelin-induced testosterone release: a biological assay for quality assurance of gonadorelin in veterinary medicine.

Two experiments were conducted with bulls administered norgestomet and gonadorelin to determine if the gonadorelin-induced release of testosterone could be developed into a biological assay for quality assurance of gonadorelin. Implants containing norgestomet (0 to 36 mg) reduced the episodic release (r = -.81; P < .05) and mean concentrations of testosterone (r = -.82; P < .05). Gonadoreline-induced testosterone release increased (r = .99; P < .05) with increasing dosage of gonadorelin (up to 5 micrograms) in norgestomet-implanted bulls (36 mg). Maximal testosterone was released (> sixfold increase) with 5 to 40 micrograms of gonadorelin. In summary, the gonadorelin-induced testosterone release in bulls administered a synthetic progestin is a sensitive (0.008 microgram per kg body weight for 5 micrograms of gonadorelin) biological assay with a rapid turnaround time for the confirmation of gonadorelin potency. Based on a per-kg-body-weight basis, the norgestomet-treated bull is the most sensitive biological assay model.
AuthorsD J Kesler, R N Summers, C A Peterson, T L Steckler
JournalDrug development and industrial pharmacy (Drug Dev Ind Pharm) Vol. 25 Issue 3 Pg. 315-21 (Mar 1999) ISSN: 0363-9045 [Print] UNITED STATES
PMID10071824 (Publication Type: Journal Article)
Chemical References
  • Drug Implants
  • Pregnenediones
  • Progestins
  • Gonadotropin-Releasing Hormone
  • norgestomet
  • Testosterone
  • Animals
  • Biological Assay (veterinary)
  • Cattle
  • Drug Implants
  • Gonadotropin-Releasing Hormone (pharmacology, standards)
  • Male
  • Pregnenediones (pharmacology)
  • Progestins (pharmacology)
  • Quality Control
  • Testosterone (metabolism)
  • Time Factors

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