A putative sigma factor from Streptomyces sp. strain A21 can activate the expression of the cryptic operon bgl in Escherichia coli K-12.

Abstract

Streptomyces sp A21 is a cellulolytic strain isolated from soil which was assigned to the genus Streptomyces on the basis of distinctive morphological features. A genomic library of A21 DNA has been constructed and transformed into Escherichia coli K-12 using a high-copy-number vector. One of the recombinant plasmids activates the cryptic bgl operon when inserted into appropriate strains. The complete sequence of the 1629-bp A21 DNA fragment has been determined. The analysis revealed the presence of an ORF whose putative product shows a high degree of similarity to RNA polymerase sigma factors; we therefore designated the gene psfS (Putative sigma factor, Streptomyces). Mapping of the 5' terminus of transcript by primer extension indicated that PsfS induces transcription initiation within the bgl promoter-silencer region.

• Authors: L Marri, S Daly, B Perito, E Tamburini, G Mastromei
• Journal: Molecular & general genetics : MGG (Mol Gen Genet) Vol. 261 Issue 1 Pg. 201-7 (Feb 1999) ISSN: 0026-8925 [Print] Germany
• PMID: 10071227 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• DNA, Bacterial
• Glucosides
• PSFS protein, Streptomyces sp. A21
• Sigma Factor
• beta-Glucosidase

Topics

• Amino Acid Sequence
• Bacterial Proteins
• DNA, Bacterial (chemistry, genetics)
• Escherichia coli (enzymology, genetics)
• Gene Expression Regulation, Bacterial
• Gene Expression Regulation, Enzymologic
• Glucosides (metabolism)
• Molecular Sequence Data
• Operon
• Plasmids (genetics)
• Promoter Regions, Genetic
• Sequence Homology, Amino Acid
• Sigma Factor (genetics)
• Streptomyces (genetics)
• Transformation, Genetic
• beta-Glucosidase (genetics, metabolism)