Abstract |
Experimental autoimmune neuritis (EAN) is a CD4(+)T cell-mediated demyelinating disease of the peripheral nervous system (PNS) and serves as an experimental model for human immune-demyelinating neuropathies. In this study, we examined the effect of recombinant rat interleukin-4 (rrIL-4) on chronic EAN in Lewis rats induced by immunization with P0 peptide 180-199 and complete Freund's adjuvant (CFA). We estimated that nasal administration of rrIL-4, in dose ranges of 0.1-1 microg/rat/day in the initial phase of EAN, decreased the severity and the duration of clinical EAN. Hyporesponsiveness of T cells, downregulation of Th1 cell responses (INF-gamma), but increased levels of specific IgG1 isotypes document that nasal administration of rrIL-4 was systemically immune effective. Low grade inflammation and complete lack of regional demyelination within the sciatic nerves were seen in rrIL-4 treated rats. Based on these observations we suggest that nasal administration of IL-4 could be further evaluated, considering its possible use in human immune-demyelinating neuropathies.
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Authors | G Deretzi, L P Zou, S H Pelidou, I Nennesmo, M Levi, B Wahren, E Mix, J Zhu |
Journal | Journal of autoimmunity
(J Autoimmun)
Vol. 12
Issue 2
Pg. 81-9
(Mar 1999)
ISSN: 0896-8411 [Print] England |
PMID | 10047428
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 1999 Academic Press. |
Chemical References |
- Immunoglobulin G
- Recombinant Proteins
- Interleukin-4
- Interferon-gamma
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Topics |
- Administration, Intranasal
- Animals
- Autoimmune Diseases
(immunology, pathology, therapy)
- Demyelinating Diseases
(immunology, pathology, therapy)
- Disease Models, Animal
- Humans
- Immunoglobulin G
(blood, classification)
- Interferon-gamma
(metabolism)
- Interleukin-4
(administration & dosage)
- Lymphocyte Activation
- Male
- Neuritis
(immunology, pathology, therapy)
- Rats
- Rats, Inbred Lew
- Recombinant Proteins
(administration & dosage)
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