Abstract |
To clarify the significance of the expression of pyrimidine nucleoside phosphorylase (PyNPase) mRNA as a predictive factor for the prognosis of patients with oesophageal carcinoma, the PyNPase mRNA in the tumours and normal tissues from 55 resected cases of oesophageal carcinoma was examined by a reverse transcription polymerase chain reaction (RT-PCR). As a result, a positive correlation was observed between the tumour/normal (T/N) ratio of the expression of PyNPase mRNA by RT-PCR and that of the enzyme activity of PyNPase based on the findings of an enzyme linked immunosolvent assay (r = 0.594, P = 0.009). The T/N ratio of the expression of PyNPase mRNA was significantly higher in the cases with lymph vessel invasion (P = 0.013), lymph node metastasis (P = 0.0016), and an advanced stage of the disease (P = 0.021) than those without these factors. The patients with a higher T/N ratio of PyNPase mRNA showed significantly worse prognosis than those with a lower T/N ratio (P = 0.023 with log-rank tests). A multivariate analysis for the cumulative survival rates revealed that a high T/N ratio of the expression of PyNPase mRNA was independently related to a poor prognosis. These findings suggested that the determination of PyNPase mRNA by RT-PCR thus appears to be a new useful parameter for identifying both a poor prognosis and a highly malignant potential of oesophageal carcinoma.
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Authors | M Yamagata, M Mori, K Mimori, K I Mafune, Y Tanaka, H Ueo, T Akiyoshi |
Journal | British journal of cancer
(Br J Cancer)
Vol. 79
Issue 3-4
Pg. 565-9
(Feb 1999)
ISSN: 0007-0920 [Print] England |
PMID | 10027331
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- RNA, Messenger
- Pentosyltransferases
- Pyrimidine Phosphorylases
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(analysis, genetics)
- Carcinoma
(genetics, pathology)
- Esophageal Neoplasms
(genetics, pathology)
- Female
- Humans
- Male
- Middle Aged
- Neoplasm Invasiveness
- Neoplasm Metastasis
- Pentosyltransferases
(biosynthesis, genetics)
- Prognosis
- Pyrimidine Phosphorylases
- RNA, Messenger
(biosynthesis)
- Reverse Transcriptase Polymerase Chain Reaction
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