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Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase

An amidohydrolase that removes intact asparagine-linked oligosaccharide chains from glycoproteins. It requires the presence of more than two amino-acid residues in the substrate for activity. This enzyme was previously listed as EC 3.2.2.18.
Also Known As:
Glycopeptidase A; Glycopeptidase F; Glycopeptide N-glycosidase; N-Glycanase; N-Glycosidase A; N-Glycosidase F; N-Oligosaccharide Glycopeptidase; PNGase A; PNGase F; Peptide N-Glycanase; Peptide N-Glycosidase; Peptide N-glycohydrolase F; Peptide-N(4)-(acetyl-beta-glucosaminyl)Asparagine Amidase; Peptide-N-Glycanase; Glycopeptidase, N-Oligosaccharide; Glycopeptide N glycosidase; N Glycanase; N Glycosidase A; N Glycosidase F; N Oligosaccharide Glycopeptidase; N-Glycanase, Peptide; N-Glycosidase, Peptide; N-glycohydrolase F, Peptide; N-glycosidase, Glycopeptide; Peptide N Glycanase; Peptide N Glycosidase; Peptide N glycohydrolase F; Glycopeptidase
Networked: 41 relevant articles (2 outcomes, 0 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1. Tickotsky-Moskovitz, Nili: 1 article (11/2015)
2. Schmidt, Mary: 1 article (09/2015)
3. Mirzaei, Parvin: 1 article (09/2015)
4. Moskal, Joseph R: 1 article (09/2015)
5. Wildburger, Norelle C: 1 article (09/2015)
6. Lang, Frederick F: 1 article (09/2015)
7. Zhou, Shiyue: 1 article (09/2015)
8. Kroes, Roger A: 1 article (09/2015)
9. Gumin, Joy: 1 article (09/2015)
10. Mechref, Yehia: 1 article (09/2015)

Related Diseases

1. Neoplasms (Cancer)
01/01/1996 - "N-Glycanase treatment reduced this apparent MW to about 45 kDa. Together, these data suggest that normal and tumor pancreatic cells contain a specific GRP receptor that is expressed more on malignant pancreatic tissues."
10/14/2012 - "In the tumor metastasis assay (in vivo), average lymph node weights of the untreated Hca-F group compared with treated Hca-F groups (tunicamycin 5 μg/mL: 0.84 ± 0.21 g vs 0.72 ± 0.19 g, P = 0.3237; 10 μg/mL: 0.84 ± 0.21 g vs 0.54 ± 0.11 g, P = 0.0113; 20 μg/mL: 0.84 ± 0.21 g vs 0.42 ± 0.06 g, P = 0.0008); (PNGase F 8 h: 0.79 ± 0.15 g vs 0.63 ± 0.13 g, P = 0.0766; 16 h: 0.79 ± 0.15 g vs 0.49 ± 0.10 g, P = 0.0022; 24 h: 0.79 ± 0.15 g vs 0.39 ± 0.05 g, P = 0.0001). "
09/04/2015 - "Orthogonal validation with topical PNGase F deglycosylation on the tumor regions of xenograft tissue, followed by nLC-ESI-MS, confirmed the presence of increased high mannose type N-glycans in the non-attractors. "
09/05/1990 - "A 36-kDa binding protein (BP), not digestible with N-glycanase, is increased in patients with extrapancreatic tumor hypoglycemia and during IGF I administration in healthy adults. "
10/14/2012 - "Western blotting was used to analyze the expression profile of Axl glycoprotein in mouse hepatocellular carcinoma cell line Hca-F treated with tunicamycin and PNGase F 3-(4,5)-dimethylthiazol(-zyl)-3,5-diphenyltetrazolium bromide (MTT) assay, extracellular matrix (ECM) invasion assay (in vitro) and tumor metastasis assay (in vivo) were utilized to evaluate the effect of Axl deglycosylation on the Hca-F cell proliferation, invasion and lymphatic metastasis. "
2. Septic Shock (Toxic Shock Syndrome)
3. Infection
4. Hepatocellular Carcinoma (Hepatoma)
04/01/1993 - "Incubation of the mature normal rat hepatocyte beta 1-subunit with peptide N-glycosidase reduced its molecular mass from 115 kDa to 82 kDa, as judged by SDS/PAGE, while the molecular mass of the abnormal mature Zajdela hepatoma cell beta 1-subunit decreased from 130 to 110 kDa. Thus, in addition to alterations in the Asn-linked oligosaccharide processing, 'ascitic growth' induced other post-translational modifications in the Zajdela hepatoma cell beta 1-subunit. "
04/01/1993 - "Digestion of both the normal rat hepatocytes and Zajdela hepatoma cells 100-kDa beta 1-precursors with endo-beta-N-acetylglucosaminidase H and peptide N-glycosidase yielded products from 100 kDa to 84 kDa and 82 kDa, respectively, as judged by SDS/PAGE, suggesting that the same polypeptide chain is synthesized in normal rat hepatocytes and in Zajdela hepatoma cells. "
06/01/1996 - "The electrophoretic profiles on sodium dodecyl sulfate-polyacrylamide get electrophoresis both before and after glycopeptidase F treatment were indistinguishable from those of a hepatoma AFP. "
10/14/2012 - "Western blotting was used to analyze the expression profile of Axl glycoprotein in mouse hepatocellular carcinoma cell line Hca-F treated with tunicamycin and PNGase F 3-(4,5)-dimethylthiazol(-zyl)-3,5-diphenyltetrazolium bromide (MTT) assay, extracellular matrix (ECM) invasion assay (in vitro) and tumor metastasis assay (in vivo) were utilized to evaluate the effect of Axl deglycosylation on the Hca-F cell proliferation, invasion and lymphatic metastasis. "
03/22/1996 - "Transduction of cultured Hepa (mouse hepatoma) cells and LDLR-deficient CHO-ldlA7 cells in vitro by the virus led to high-level expression of immunoreactive VLDLR proteins with molecular sizes of 143 kDa and 161 kDa. Digestion of the cell extract with the enzymes neuraminidase, N-glycanase, and O-glycanase resulted in the stepwise lowering of the apparent size of the 161-kDa species toward the 143-kDa species. "
5. Melanoma (Melanoma, Malignant)

Related Drugs and Biologics

1. Antigens
2. ABO Blood-Group System
3. keratanase II
4. keratan-sulfate endo-1,4-beta-galactosidase
5. Proteins (Proteins, Gene)
6. Polysaccharides
7. Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
8. Tunicamycin
9. Mannose (D-Mannose)
10. Proteasome Endopeptidase Complex (Proteasome)

Related Therapies and Procedures

1. Heterologous Transplantation (Xenotransplantation)