Peptide Elongation Factor G (EF G)

12/02/2019 - "We previously measured a power stroke accompanying EF-G catalysis, which was consistent with structural studies. "
12/02/2019 - "Modulation and Visualization of EF-G Power Stroke During Ribosomal Translocation."
01/01/2016 - "Elongation factor G initiates translocation through a power stroke."
12/02/2019 - "The established force -structure-function correlation for EF-G indicates that power stroke drives ribosomal translocation, but the mRNA reading frame is probably maintained by ribosome itself. "
10/01/2014 - "EF-G combines the energy regimes of a GTPase and a motor protein and facilitates tRNA movement by a combination of directed Brownian ratchet and power stroke mechanisms. "

07/01/2000 - "We show that fusidic acid-resistant EF-G mutants have perturbed ppGpp basal levels during steady-state growth and perturbed induced levels during starvation. "
01/01/1981 - "However, EF-G is probably not required for the synthesis of ppGpp during the stringent response, since its inactivation did not prevent ppGpp accumulation during amino acid starvation."
01/01/2016 - "Furthermore, 14 unique proteins in 18 spots of the surface subproteome underwent changes identified by differential 2DE, including elongation factor G, thermostable pullulanase, and phosphate starvation inducible stress-related protein increasing in a range of +2.1 - +4.7 fold. "
12/22/1976 - "EF-Ts, L12 and S6 were found to be under strong stringent and relaxed regulation; EF-G, EF-Tu-A and S1 are under strong stringent, but weak relaxed regulation; EF-Tu-B, alpha, VRS, IRS and ARS are under week stringent and weak relaxed regulation; beta is under weak stringent regulation and does not respond at all to relaxed conditions; the biosynthetic rate of a protein called stringent starvation protein is strongly stimulated, relative to other proteins, in the starved stringent strain."
01/01/2017 - "acidophilus NCFM had phosphate starvation inducible protein stress-related, thermostable pullulanase, and elongation factor G increasing 4.4-2.4 fold, while GAPDH, elongation factor Ts, and pyruvate kinase were reduced by 2.0-1.5 fold in relative abundance. "

03/01/1994 - "Using high-resolution two-dimensional gel electrophoresis and specific immunoprecipitation, we show that elongation factor G and ribosomal protein S6 are phosphorylated following T7 infection. "
03/30/1976 - "Following infection of ultraviolet light-irradiated Escherichia coli with either lambdadfus-3 or lambdadrifd18, the EF-Tu gene, tufA, near 65 minutes on the genetic map is expressed as 3-4 copies per EF-G molecule. "
09/12/1975 - "(1) In CP78G carrying temperature-sensitive elongation factor G, shift-up to high temperature (41 degrees C) in the middle of phage infection did not affect early mRNA synthesis with lambdatof+ phage but did inhibit it with lambdatof- phage. "
01/01/2003 - "Fusidic acid is a potent antibiotic against severe Gram-positive infections that interferes with the function of elongation factor G (EF-G), thereby leading to the inhibition of bacterial protein synthesis. "

07/01/1999 - "Upon GTP hydrolysis, the bifurcation is reversed and an arc-like connection is formed between the base of the stalk and EF-G."
12/09/2005 - "During tRNA translocation on the ribosome, an arc-like connection (ALC) is formed between the G' domain of elongation factor G (EF-G) and the L7/L12-stalk base of the large ribosomal subunit in the GDP state. "
08/24/2001 - "Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. "

04/01/2006 - "Using a sophisticated extension of the PCR technique, we have successfully isolated two single-copy nuclear genes, DMC1 and EF-G, from each of the three genomes found in hexaploid wheat (BA(u)D) and from the two genomes of the tetraploid progenitor Triticum turgidum (BA(u)). "
01/01/2006 - "Sequences from three nuclear regions (DMC1, EF-G and ITS) were combined and analysed phylogenetically for all diploid (20 species) and two tetraploid Hordeum species and the outgroup Psathyrostachys. "