Trypan Blue

A diazo-naphthalene sulfonate that is widely used as a stain.

Also Known As:

Diamine Blue; Niagara Blue; VisionBlue; Blue, Diamine; Blue, Niagara; Blue, Trypan; 2,7-Naphthalenedisulfonic acid, 3,3'-((3,3'-dimethyl(1,1'-biphenyl)-4,4'-diyl)bis(azo))bis(5-amino-4-hydroxy-), tetrasodium salt

Networked: 1367 relevant articles (37 outcomes, 104 trials/studies)

Relationship Network

Drug Context: Research Results

Experts

1.	Rodrigues, Eduardo B: 7 articles (11/2020 - 01/2004)
2.	Cho, Sung-Dae: 7 articles (06/2019 - 12/2014)
3.	Shin, Ji-Ae: 7 articles (06/2019 - 12/2014)
4.	Maia, Mauricio: 6 articles (11/2020 - 01/2007)
5.	Meyer, Carsten H: 6 articles (11/2009 - 01/2004)
6.	Farah, Michel E: 5 articles (11/2020 - 05/2007)
7.	Karami, Hadi: 5 articles (11/2019 - 01/2013)
8.	Yang, In-Hyoung: 5 articles (06/2019 - 12/2014)
9.	Haritoglou, Christos: 5 articles (04/2013 - 07/2004)
10.	Penha, Fernando M: 5 articles (09/2009 - 05/2007)

Related Diseases

1.	Cataract (Cataracts) 05/01/2004 - "Cataract surgery using trypan blue was safe and effective in managing white cataracts." 09/01/2005 - "To assess the safety and efficacy of Manual Small Incision Cataract Surgery (MSICS) in cases of white cataract with the use of trypan blue as an adjunct for performing continuous curvilinear capsulorthexis (CCC). " 01/01/2022 - "Use of trypan blue in paediatric cataract surgery probably leads to better outcomes, but more well-conducted randomised controlled trials on this important topic are needed." 06/01/2009 - "The purpose of the study was to evaluate the effect of trypan blue on intraocular pressure (IOP) after small-incision cataract surgery. " 03/01/2001 - "Twenty patients with mature cataract were enrolled in the study and opereted on after injection of trypan blue in the anterior chamber. "
2.	Neoplasms (Cancer) 07/01/2021 - "tinctorum resulted in significant reduction in the cell viabilities of cancer cells with IC50 values ranging between 1.2 and 12.8 μg/ml. The potential anticancer effect of the bioactive fraction was further supported by Trypan blue cell viability, LDH and DNA fragmentation assays. " 05/01/2023 - "The design, molecular docking, synthesis and structure-activity relationship (SAR) of a series of novel methyl 1-oxo-2-(propan-2-yl)-3-(pyridin-3-yl)-1,2,3,4-tetrahydroisoquinoline-4-carboxylates, were investigated for antiproliferative and cytotoxic studies by screening against cancer cell lines of different origin by MTT, LDH and Trypan Blue Assay. " 12/08/2020 - "Promising effective synergistic results have been demonstrated through in vitro study in multi-model cancer cell lines via both trypan blue exclusion and bioluminescence imaging methods. " 01/01/2011 - "MTT assay and the trypan blue exclusion method were used for the in vitro study, while tumor size and the SUV of the 18FDG-PET scans were measured in animal models. " 10/01/1985 - "Trypan blue studies indicated that less than 1% of the cells frozen to -35 degrees C were considered viable, yet significant tumor take rates were noted, suggesting that for some cells the cryoinjury is reversible. "
3.	Hypoxia (Hypoxemia) 06/01/2021 - "For evaluating the effect of mimic hypoxia on proliferation of MOLT-4 and KG1 cell lines, cell counting was done using trypan blue at 24, 48, and 72 hours. " 01/01/2020 - "Trypan blue exclusion assay and Transwell and flow cytometry assays were conducted to assess hypoxia-induced injury by determining the viability, migration, invasion, and apoptosis of H9c2 cells in different conditions, respectively. " 01/01/2019 - "Hypoxia-induced injury was investigated by cell viability and apoptosis using the trypan blue exclusion method, flow cytometry and Western blot. " 03/15/2016 - "To evaluate the effect of hypoxia on PMSCs, proliferation of PMSCs was detected by trypan blue staining and the HIF-1α level was detected by western blot. " 03/01/2014 - "The preconditioning was proved by trypan blue cell counts following 5 h of hypoxia and confirmed by fluorescence staining. "
4.	Epiretinal Membrane (Epiretinal Membranes) 06/01/2011 - "The purpose of this study was to evaluate "in vivo" safety of trypan blue (TB) in patients undergoing TB-assisted internal limiting membrane or epiretinal membrane peeling. " 04/01/2010 - "The purpose of this study was to correlate the ultrastructural morphology of epiretinal tissue with optical coherence tomography and to investigate the effects of trypan blue staining on epiretinal membrane (ERM) ultrastructure and clinical outcome. " 02/01/2005 - "[Ultrastructural study of epiretinal membrane stained by trypan blue: 15 case reports]." 04/01/2014 - "Trypan blue stains epiretinal membranes and facilitates their complete removal. " 04/01/2013 - "To evaluate the selectivity and strength of intraoperative trypan blue staining during removal of epiretinal membranes (ERMs) and the internal limiting membrane. "
5.	Infections 01/01/2017 - "The median lethal dose (LD50) was 40 μg/mL and no morphological changes were observed by staining with blue trypan and light microscopy; experimental gerbil infection was eliminated with 320 μg/Kg of weight. " 02/05/2020 - "The degree of severity of the leaf lesions and the results of trypan blue staining showed that the plants overexpressing Csn-miR477 exhibited more severe damage upon pathogen infection than wild-type plants. " 07/01/2019 - "Positive microbiology and genomic DNA typing results proved that the contaminated trypan blue solutions were the source of infection in this outbreak. " 07/01/2019 - "The virus titers in the liquid supernatant of the above three groups of cells were detected on different time, and the mortality of cells of each group was tested with trypan blue exclusion test at 24 h post VSV infection. " 01/15/2018 - "Trypan blue staining determined BOEC viability during 24 h co-culture with different multiplicities of infection (MOI) of T. pyogenes (MOI 0.01, 0.05, 0.1 and 1) or B. pumilus (MOI 1 and 10). "

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4.	Annexin A5
5.	Proteins (Proteins, Gene)
6.	Adenosine Triphosphate (ATP)
7.	Bromides
8.	Triamcinolone Acetonide (Azmacort)
9.	Methylene Blue (Methylthioninium Chloride)
10.	DNA Nucleotidylexotransferase (Terminal Deoxynucleotidyl Transferase)

Related Therapies and Procedures

1.	Phacoemulsification
2.	Vitrectomy
3.	Capsulorhexis
4.	Vitreoretinal Surgery
5.	Intravenous Injections