|1.||Owen, Jonathan P: 2 articles (10/2007 - 02/2007)|
|2.||Whitelam, Garry C: 2 articles (10/2007 - 02/2007)|
|3.||Maddison, Ben C: 2 articles (10/2007 - 02/2007)|
|4.||Gough, Kevin C: 2 articles (10/2007 - 02/2007)|
|5.||Hanai, N: 2 articles (10/2000 - 08/2000)|
|6.||Suzawa, T: 2 articles (10/2000 - 08/2000)|
|7.||Ohta, S: 2 articles (10/2000 - 08/2000)|
|8.||Saito, H: 2 articles (10/2000 - 08/2000)|
|9.||Nagamura, S: 2 articles (10/2000 - 08/2000)|
|10.||Yamasaki, M: 2 articles (10/2000 - 08/2000)|
05/01/1995 - "Site-directed mutagenesis studies of the thermolysin-like proteinase (TLP) from Bacillus stearothermophilus (TLP-ste) show that its reduced resistance to thermally induced autolysis, as compared to thermolysin, is due to only some of the 44 naturally occurring amino-acid differences between them. "
12/01/1986 - "High molecular weight autolysis products from thermolysin have been isolated and identified. "
04/22/1986 - "Limited proteolysis or autolysis of thermolysin under different experimental conditions leads to fission of a small number of peptide bonds located in exposed surface segments of the polypeptide chain characterized by highest mobility, as given by the temperature factors (B values) determined crystallographically [Holmes, M.A., & Matthews, B.W. "
04/15/1986 - "Autolysis of thermolysin. "
10/01/1986 - "Equilibrium and kinetic studies of the unfolding and autolysis of the two domain protein thermolysin in guanidine hydrochloride are described. "
08/01/2000 - "DU-257 was condensed to the PEG-dipeptidyl linker through carboxyl terminal of dipeptide, and enzymatic release of DU-257 using a model enzyme, thermolysin, similar enzyme of which was shown to be overexpressed at various tumor sites, was evaluated by HPLC analysis. "
01/01/1983 - "By fingerprint analysis of the CEA digested with thermolysin, we could demonstrate differences between the two lung tumor CEA bands and the colonic tumor CEA. "
05/01/2008 - "Thermolysin treatment of tumor tissue liberated single cells from the tissue and allowed infection with ISVPs, but this did not result in the production of infectious virus particles. "
10/03/2000 - "When segment B was derivatized to have an amino residue and then linked to PEG through a dipeptide, segment B was cleaved at the peptide bond by a particular enzyme, thermolysin (EC 188.8.131.52), but not by plasmin (EC 3.4.2 1.7.), indicating that certain protease specifically expressed at the tumor site would be capable of peptide-specific digestion and release of anti-tumor agent since a thermolysin-like enzyme has been reported to be expressed at many tumor cells. "
|3.||Prion Diseases (Transmissible Spongiform Encephalopathies)
10/01/2007 - "Molecular profiling of ovine prion diseases by using thermolysin-resistant PrPSc and endogenous C2 PrP fragments."
02/01/2007 - "Here, we have applied the protease thermolysin to the diagnosis of animal prion diseases. "
02/01/2007 - "Use of thermolysin in the diagnosis of prion diseases."
12/01/2008 - "Detection of PK-sensitive isoforms of disease-related PrP using thermolysin should be useful for improving diagnostic sensitivity in human prion diseases."
|4.||Bovine Spongiform Encephalopathy (Mad Cow Disease)
12/01/2008 - "In vCJD (variant Creutzfeldt-Jakob disease), the human counterpart of BSE (bovine spongiform encephalopathy), up to 90% of total PrP present in the brain resists degradation with thermolysin, whereas only approximately 15% of this material resists digestion by PK. "
10/01/2007 - "Here we describe a novel molecular strain typing assay that used thermolysin digestion of caudal medulla samples to produce PrPres signatures on Western blots that readily distinguished experimental sheep bovine spongiform encephalopathy (BSE) from classical scrapie. "
02/01/2007 - "Thermolysin was used in the diagnosis of ovine scrapie and bovine spongiform encephalopathy and produced comparable assay sensitivity to assays using proteinase K digestion. "
07/01/1994 - "MDL 101,146 had no effect on thermolysin-induced pulmonary hemorrhage, which demonstrated the specificity of MDL 101,146 for HNE in vivo. "
03/01/1990 - "Lung hemorrhage could also be induced by intratracheal instillation of other elastolytic enzymes, such as thermolysin, and inhibition of hemorrhage was seen only with inhibitors active against the type of elastase used. "
01/01/2011 - "The IMPI exhibits a specific and potent activity against thermolysin-like microbial metalloproteinases including a number of prominent virulence and/or pathogenic factors of human pathogens which are responsible for severe symptoms such as septicemia, hemorrhagic tissue bleeding, necrosis and enhancement of vascular permeability. "
03/01/1990 - "Methoxysuccinyl-alanyl-alanyl-prolyl-valine-chloromethylketone (MeOSuc-AAPV-CMK), as well as alpha 1-proteinase inhibitor (alpha 1PI) but not tosyl-lysine-chloromethylketone (tosyl-lysine-CMK), inhibited the hemorrhage caused by human PMN elastase, but the specific inhibitors of this enzyme had no effect on thermolysin-induced lung hemorrhage. "
|1.||Guanidine (Guanidine Nitrate)
|2.||Virulence Factors (Pathogenicity Factors)
|6.||Edetic Acid (EDTA)
|9.||Protein Isoforms (Isoforms)
|10.||Endopeptidase K (Proteinase K)