Fixatives (Pickling Agents)

Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.

Also Known As:

Networked: 439 relevant articles (11 outcomes, 46 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Bibeau, Frédéric: 3 articles (01/2013 - 05/2006)
2.	Sebag, J: 3 articles (01/2008 - 07/2002)
3.	Boon, Mathilde E: 3 articles (06/2007 - 09/2005)
4.	Nishikawa, Takeshi: 2 articles (06/2022 - 01/2021)
5.	Ohbayashi, Chiho: 2 articles (06/2022 - 10/2005)
6.	Chidlow, Glyn: 2 articles (08/2021 - 10/2011)
7.	Engel, Kelly B: 2 articles (01/2019 - 05/2011)
8.	Moore, Helen M: 2 articles (01/2019 - 05/2011)
9.	Giaccone, G: 2 articles (08/2018 - 01/2000)
10.	Tagliavini, F: 2 articles (08/2018 - 01/2000)

Related Diseases

1.	Dehydration (Water Stress) 12/01/2022 - "The current structural models of the mammalian SC are based on electron microscopy, superresolution, and expansion microscopy studies using chemical fixatives and sample dehydration of gonads, which are methodologies known to produce structural artifacts. " 02/28/1999 - "Until now, most ultrastructural studies on the neuromuscular junction have been carried out on samples first exposed to chemical treatments--with fixatives and/or dehydration agents--that are known to induce, or to be inadequate to prevent, artefactual changes of the native state. " 01/01/1989 - "In this study we concentrate on the influence of fixatives, commonly used in immuno-electron microscopy, the dehydration process, and the embedding media Lowicryl HM20 and K4M. " 04/28/2023 - "The amount of residual water depended primarily on the fixative type and the dehydration protocol and, to a lesser extent, on storage conditions and time. " 02/03/2023 - "Pre-analytical factors such as fixative temperature, dehydration, and embedding media affect downstream analysis, generating data dependent on technical processing rather than disease state. "
2.	Cysts 09/01/2009 - "The comparison of the efficacy of various fixatives on diverse staining methods of Giardia lamblia cyst." 12/01/2018 - "The aim of the present study was to identify Blastocystis spp. (particularly the granular form), Dientamoeba fragilis, and Entamoeba histolytica/dispar trophozoites, which are difficult to identify using wet mount, as well as Giardia intestinalis cysts, which are difficult to identify by unexperienced researchers, in fresh fecal samples within 5 min using a different fixative employing trichrome staining. " 09/01/2009 - "After the smears of stool samples containing Giadria lamblia cyst were prepared and kept for 24 h in various fixatives, the study was carried out using the four above-mentioned stains by changing the ingredients and time as well as by repeating the experiments. " 01/01/1989 - "Three of the fixatives employed gave good fixation of cysts and intracystic bodies, but for the trophic forms fixation was only fair. " 02/01/1995 - "Four hundred forty-five bronchoalveolar lavage specimens from patients with the human immunodeficiency virus were preserved in Saccomano's fixative and stained for Pneumocystis carinii cysts by a modified method with Fungi-Fluor Solution A (Polysciences, Warrington, Pa) and the Genetic Systems Pneumocystis carinii Immunofluorescence Antibody (Genetic Systems Corp, Seattle, Wash). "
3.	Cataract (Cataracts) 07/01/2009 - "Combined microwave energy and fixative agent for cataract induction in pig eyes." 07/01/2012 - "Experienced cataract surgeons who performed CCC on these rabbit models strongly agreed that isolated rabbit lenses treated with fixative provide a realistic simulation of CCC in human patients and that both models were useful tools for capsulorhexis instruction. " 01/01/1986 - "During studies of morphologic changes in various experimentally induced cataracts, an improved procedure was developed, utilizing isomolar fixative and water-soluble embedding medium (glycol methacrylate), while avoiding dehydration with organic solvents. "
4.	Basal Cell Carcinoma (Rodent Ulcer) 02/01/2009 - "This study was designed to evaluate the efficacy of different fixatives, of microwaving and microwave pretreatment method to retrieve p53 immunoreactivity in paraffin-embedded non-lesioned (adjacent normal tissue) human skin samples or pathological human skin samples diagnosed as basal cell carcinoma. "
5.	Rupture 05/01/2006 - "Morphological anomalies, such as membrane rupture, detached tails, and the formation of tail vesicles (typically associated with damage attributable to procedures such as cryopreservation) were observed; the Mantel-Haenszel Chi-square test indicated a strong association between the anomalies and fixative osmolality (P<0.001). " 01/01/1982 - "Fibrin glue turned out to be a valuable additional fixative, effective in preventing ruptures of the anastomoses. " 01/01/2017 - "The latter most likely cause artificial ruptures of delicate chitin lamellae in the inner of the chaeta when treated with acidic fixatives. " 06/01/2008 - "Staining of Pf nucleic acids allows for analysis of parasite development in the absence of fixatives, lysis, or radioactivity to enable examination of erythrocytes from parasite invasion through schizont rupture using sensitive and rapid assay procedures. " 11/01/1993 - "However, when fixed in hypotonic fixative to rupture plasma membranes, or when ruptured after fixation in normotonic fixative, the cytoplasmic surfaces of presynaptic membranes and synaptic vesicles were labelled by gold particles. "

Related Drugs and Biologics

1.	Formaldehyde (Formol)
2.	Paraffin
3.	Proteins (Proteins, Gene)
4.	DNA (Deoxyribonucleic Acid)
5.	hydroxyethyl methacrylate (HEMA)
6.	Solvents
7.	Isomolar
8.	Copper
9.	Fibrin Tissue Adhesive (Fibrin Sealant)
10.	Calcium Oxalate

Related Therapies and Procedures

1.	Fluid Therapy (Oral Rehydration Therapy)
2.	Neoadjuvant Therapy
3.	Cryotherapy (Therapy, Cold)
4.	Sutures (Suture)
5.	Surgical Instruments (Clip)